Abstract
Twelve hyper-β carotene-producing strains of algae assigned to the genus Dunaliella salina have been isolated from various hypersaline environments in Israel, South Africa, Namibia and Spain. Intron-sizing of the SSU rDNA and phylogenetic analysis of these isolates were undertaken using four commonly employed markers for genotyping, LSU rDNA, ITS, rbcL and tufA and their application to the study of Dunaliella evaluated. Novel isolates have been identified and phylogenetic analyses have shown the need for clarification on the taxonomy of Dunaliella salina. We propose the division of D. salina into four sub-clades as defined by a robust phylogeny based on the concatenation of four genes. This study further demonstrates the considerable genetic diversity within D. salina and the potential of genetic analyses for aiding in the selection of prospective economically important strains.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 27-37 |
| Number of pages | 11 |
| Journal | Journal of the Marine Biological Association of the United Kingdom |
| Volume | 101 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 2021 |
Bibliographical note
Funding Information:This research was supported by the D-factory and the EU KBBE.2013.3.2-02 programme (D-Factory: 368 613870) and the ValgOrize consortium, subsidised by the Interreg 2 Seas programme 2014–2020, co-financed by the European Fund for Regional Development in the framework of subsidy contract nr. 2S05-17.
Publisher Copyright:
Copyright © The Author(s), 2021. Published by Cambridge University Presson behalf of Journal of the Marine Biological Association of the United Kingdom.
Keywords
- Diversity
- Dunaliella salina
- ITS
- LSU
- SSU
- phylogeny
- rbcL
- tufA
