Molecular phylogeny of the marine sand-dwelling dinoflagellate herdmania litoralis and an emended description of the closely related planktonic genus archaeperidinium jörgensen

Aika Yamaguchi, Mona Hoppenrath, Vera Pospelova, Takeo Horiguchi, Brian S. Leander

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Herdmania litoralis is a heterotrophic, sand-dwelling dinoflagellate with morphological characters that do not provide clear evidence for its systematic position in any existing family of dinoflagellates. Protoperidinium minutum is a heterotrophic, planktonic species that has a typical tabulation for the genus Protoperidinium. In order to infer the phylogenetic positions of these two species more confidently, we characterized the thecal plate patterns and determined small-subunit and large-subunit ribosomal DNA sequences (SSU rDNA and LSU rDNA, respectively) from both species. Intraindividual and intraspecific diversity of SSU and LSU rDNA data were characterized in H. litoralis using a combination of single-cell PCR approaches and analyses of PCR clones derived from multi-cell DNA extractions. The results of the molecular phylogenetic analyses demonstrated a novel, well-supported clade comprising both sand-dwelling species (H. litoralis and Thecadinium dragescoi) and planktonic species (P. minutum). Because the establishment of this clade also demonstrated that P. minutum is not a member of Protoperidinium, we reinstated and emended the genus Archaeperidinium Jörgensen 1912.

Original languageEnglish (US)
Pages (from-to)98-112
Number of pages15
JournalEuropean Journal of Phycology
Volume46
Issue number2
DOIs
StatePublished - May 2011
Externally publishedYes

Bibliographical note

Funding Information:
was supported by a postdoctoral research salary to A.Y. and M.H. from the Assembling the Tree of Life grant (NSF #EF-0629624) and operating funds to B.S.L. from the National Science and Engineering Research Council of Canada (NSERC 283091-09); B.S.L. is a fellow of the Canadian Institute for Advanced Research, Program in Integrated Microbial Biodiversity. This work was also partly supported by the Grant-in-Aid (16370039) from the MEXT and by the 21st Century Center of Excellence (COE) Program on ‘Neo-Science of Natural History’ (Program Leader: Hisatake Okada) at Hokkaido University financed by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.

Keywords

  • Archaeperidinium
  • Herdmania litoralis
  • Heterotrophic dinoflagellates
  • Intraspecific variability
  • LSU rDNA
  • Protoperidiniaceae
  • Protoperidinium minutum
  • SSU rDNA
  • Single-cell PCR
  • Thecadinium dragescoi

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