Molecular typing and antimicrobial susceptibility of Clostridium perfringens from broiler chickens

Saad Gharaibeh, Rami Al Rifai, Ahmad Al-Majali

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Clostridium perfringens (Cp) causes necrotic enteritis disease in commercial poultry. Antimicrobials are used to control and treat this disease and sometimes clinical outbreaks do not respond well to certain treatments. This study was designed to isolate Cp from clinical cases, type these isolates by multiplex PCR, and determine their antimicrobial susceptibility by micro-dilution method. A total of 67 Cp isolates were obtained from 155 broiler chicken flocks. All isolates were classified as type A and non-enterotoxin producers. Lincomycin, erythromycins, and tilmicosin showed very high minimal inhibitory concentration (MIC) 50 of ≥256 μg/ml. However, tylosin, amoxicillin, ampicillin, penicillin, florfenicol, danofloxacin, enrofloxacin, chlortetracycline, doxycycline, and oxytetracycline had variable MIC50 of 64, 0.5, 1, 1, 8, 4, 8, 4, 8, 0.5 μg/ml, respectively. It is recommended that Cp infections in Jordan be treated with either penicillins or tetracyclines especially amoxicillin and oxytetracycline.

Original languageEnglish (US)
Pages (from-to)586-589
Number of pages4
Issue number6
StatePublished - Dec 2010

Bibliographical note

Funding Information:
This project was supported by Deanship of Scientific Research at Jordan University of Science and Technology (Grant # 126/2006 ). We also thank Dr. Saeb El-Sukhon for providing us with the NCTC reference strains.


  • Chicken
  • Clostridium perfringens
  • Minimum inhibitory concentration
  • Typing


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