Monoaminergic changes in locus coeruleus and dorsal raphe nucleus following noradrenaline depletion

Tommaso Cassano; Silvana Gaetani; Maria Grazia Morgese; Teresa MacHeda; Leonardo Laconca; Pasqua Dipasquale; Juan Taltavull; Toni S. Shippenberg; Vincenzo Cuomo; Gabriella Gobbi

doi:10.1007/s11064-009-9928-5

Monoaminergic changes in locus coeruleus and dorsal raphe nucleus following noradrenaline depletion

Tommaso Cassano, Silvana Gaetani, Maria Grazia Morgese, Teresa MacHeda, Leonardo Laconca, Pasqua Dipasquale, Juan Taltavull, Toni S. Shippenberg, Vincenzo Cuomo, Gabriella Gobbi

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Abstract

The goal of our study was to assess the monoaminergic changes in locus coeruleus (LC) and dorsal raphe nucleus (DRN) following noradrenaline (NA) depletion. Seven days after a single N-(2-chloroethyl)-N-ethyl-2- bromobenzylamine (DSP-4) intraperitoneal administration in mice, we observed a decrease of NA in both the LC and DRN, as well as in prefrontal cortex (PFC) and hippocampus (HIPP). Moreover, an increase of serotonin (5-HT) and 5-hydroxyindolacetic acid (5-HIAA) was detected at LC level, while no change was found in DRN. DSP-4 also caused a significant decrease of dopamine (DA) tissue content in HIPP and DRN, without affecting the LC and the PFC. A decrease of DA metabolite, homovanillic acid (HVA), was found in the DRN of NA-depleted mice. These results highlight that the neurotoxic action of DSP-4 is not restricted to LC terminal projections but also involves NA depletion at the cell body level, where it is paralleled by adaptive changes in both serotonergic and dopaminergic systems.

Original language	English (US)
Pages (from-to)	1417-1426
Number of pages	10
Journal	Neurochemical Research
Volume	34
Issue number	8
DOIs	https://doi.org/10.1007/s11064-009-9928-5
State	Published - Aug 2009
Externally published	Yes

Keywords

DSP-4
Dopamine
Dorsal raphe
Locus coeruleus
Noradrenaline
Serotonin

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title = "Monoaminergic changes in locus coeruleus and dorsal raphe nucleus following noradrenaline depletion",

abstract = "The goal of our study was to assess the monoaminergic changes in locus coeruleus (LC) and dorsal raphe nucleus (DRN) following noradrenaline (NA) depletion. Seven days after a single N-(2-chloroethyl)-N-ethyl-2- bromobenzylamine (DSP-4) intraperitoneal administration in mice, we observed a decrease of NA in both the LC and DRN, as well as in prefrontal cortex (PFC) and hippocampus (HIPP). Moreover, an increase of serotonin (5-HT) and 5-hydroxyindolacetic acid (5-HIAA) was detected at LC level, while no change was found in DRN. DSP-4 also caused a significant decrease of dopamine (DA) tissue content in HIPP and DRN, without affecting the LC and the PFC. A decrease of DA metabolite, homovanillic acid (HVA), was found in the DRN of NA-depleted mice. These results highlight that the neurotoxic action of DSP-4 is not restricted to LC terminal projections but also involves NA depletion at the cell body level, where it is paralleled by adaptive changes in both serotonergic and dopaminergic systems.",

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author = "Tommaso Cassano and Silvana Gaetani and Morgese, {Maria Grazia} and Teresa MacHeda and Leonardo Laconca and Pasqua Dipasquale and Juan Taltavull and Shippenberg, {Toni S.} and Vincenzo Cuomo and Gabriella Gobbi",

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AU - Cassano, Tommaso

AU - Gaetani, Silvana

AU - Morgese, Maria Grazia

AU - MacHeda, Teresa

AU - Laconca, Leonardo

AU - Dipasquale, Pasqua

AU - Taltavull, Juan

AU - Shippenberg, Toni S.

AU - Cuomo, Vincenzo

AU - Gobbi, Gabriella

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Monoaminergic changes in locus coeruleus and dorsal raphe nucleus following noradrenaline depletion

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