Mosquito ribosomal RNA genes: Characterization of gene structure and evidence for changes in copy number during development

Yang Ja Park, Ann Marie Fallon

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

A family of nine recombinant bacteriophages containing rRNA genes from cultured cells of the mosquito, Aedes albopictus, has been characterized by restriction mapping, Southern-blotting and S1-nuclease analyses. The 18S rRNA coding region measured 1800 bp and contained a conserved Eco RI site near the 3′-end. The 28S rRNA coding region was divided into α and β sequences, comprising 1750 and 2000 bp, respectively, which were separated by a 350 bp sequence that is removed from the rRNA precursor during processing. The entire rDNA repeat unit had a minimum length of 15.6 kb, including a nontranscribed spacer region that contained a series of PvuI repeats upstream of the 18S rRNA coding sequence. During development of the mosquito, Aedes aegypti, the rRNA gene copy number per haploid genome increased from about 400 in larvae to about 1200 in adults.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalInsect Biochemistry
Volume20
Issue number1
DOIs
StatePublished - 1990

Keywords

  • Aedes aegypti
  • Aedes albopictus
  • coding regions
  • copy number
  • mosquito
  • rRNA genes
  • restriction mapping

Fingerprint Dive into the research topics of 'Mosquito ribosomal RNA genes: Characterization of gene structure and evidence for changes in copy number during development'. Together they form a unique fingerprint.

Cite this