mRNA and protein levels for GABA Aα4, α5, β1 and GABABR1 receptors are altered in brains from subjects with autism

S. Hossein Fatemi; Teri J. Reutiman; Timothy D. Folsom; Robert J. Rooney; Diven H. Patel; Paul D. Thuras

doi:10.1007/s10803-009-0924-z

mRNA and protein levels for GABA _Aα4, α5, β1 and GABA_BR1 receptors are altered in brains from subjects with autism

S. Hossein Fatemi, Teri J. Reutiman, Timothy D. Folsom, Robert J. Rooney, Diven H. Patel, Paul D. Thuras

Psychiatry & Behavioral Sciences

Research output: Contribution to journal › Article › peer-review

151 Scopus citations

Abstract

We have shown altered expression of gammaaminobutyric acid A (GABA _A) and gamma-aminobutyric acid B (GABA _B) receptors in the brains of subjects with autism. In the current study, we sought to verify our western blotting data for GABBR1 via qRT-PCR and to expand our previous work to measure mRNA and protein levels of 3 GABA _A subunits previously associated with autism (GABRα4; GABRα5; GABRβ1). Three GABA receptor subunitsdemonstrated mRNA and protein level concordance in superior frontal cortex (GABRα4, GABRα5, GABRβ1) and one demonstrated concordance in cerebellum (GABBR1). These results provide further evidence of impairment of GABAergic signaling in autism.

Original language	English (US)
Pages (from-to)	743-750
Number of pages	8
Journal	Journal of Autism and Developmental Disorders
Volume	40
Issue number	6
DOIs	https://doi.org/10.1007/s10803-009-0924-z
State	Published - Jun 2010

Bibliographical note

Funding Information:
Acknowledgments Human tissue was obtained from the NICHD Brain and Tissue Bank for Developmental Disorders; the Harvard Brain Tissue Resource Center, which is supported in part by PHS grant number R24 MH068855; the Brain Endowment Bank, which is funded in part by the National Parkinson Foundation, Inc., Miami, Florida; and the Autism Tissue Program and is gratefully acknowledged. Grant support by National Institute of Child Health and Human Development (#5R01HD052074-01A2) and 3R01HD052074-03S1 to SHF is gratefully acknowledged. Portions of this paper have been presented at the following meetings: Collegium Internationale Neuro-Psychopharma-cologicum, Munich, 2008; American College of Neuropsychophar-macology, Scottsdale AZ, 2008; University of Tehran, School of Medicine, 2008; Autism One Conference, Chicago, 2009.

Funding Information:
All experimental procedures were approved by the Institutional Review Board of the University of Minnesota School of Medicine. Postmortem blocks of BA40, BA9, and cerebella (lobar origin unknown) were obtained from the Autism Research Foundation and various brain banks (NICHD Brain and Tissue Bank for Developmental Disorders; TARF; the Harvard Brain Tissue Resource Center, which is supported in part by PHS grant number R24 MH068855; the Brain Endowment Bank, which is funded in part by the National Parkinson Foundation, Inc., Miami, Florida; and the Autism Tissue Program). The tissue

Keywords

Autism
Brain
GABBR1
GABRα4
GABRα5
GABRβ1

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title = "mRNA and protein levels for GABA Aα4, α5, β1 and GABABR1 receptors are altered in brains from subjects with autism",

abstract = "We have shown altered expression of gammaaminobutyric acid A (GABA A) and gamma-aminobutyric acid B (GABA B) receptors in the brains of subjects with autism. In the current study, we sought to verify our western blotting data for GABBR1 via qRT-PCR and to expand our previous work to measure mRNA and protein levels of 3 GABA A subunits previously associated with autism (GABRα4; GABRα5; GABRβ1). Three GABA receptor subunitsdemonstrated mRNA and protein level concordance in superior frontal cortex (GABRα4, GABRα5, GABRβ1) and one demonstrated concordance in cerebellum (GABBR1). These results provide further evidence of impairment of GABAergic signaling in autism.",

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author = "Fatemi, {S. Hossein} and Reutiman, {Teri J.} and Folsom, {Timothy D.} and Rooney, {Robert J.} and Patel, {Diven H.} and Thuras, {Paul D.}",

note = "Funding Information: Acknowledgments Human tissue was obtained from the NICHD Brain and Tissue Bank for Developmental Disorders; the Harvard Brain Tissue Resource Center, which is supported in part by PHS grant number R24 MH068855; the Brain Endowment Bank, which is funded in part by the National Parkinson Foundation, Inc., Miami, Florida; and the Autism Tissue Program and is gratefully acknowledged. Grant support by National Institute of Child Health and Human Development (#5R01HD052074-01A2) and 3R01HD052074-03S1 to SHF is gratefully acknowledged. Portions of this paper have been presented at the following meetings: Collegium Internationale Neuro-Psychopharma-cologicum, Munich, 2008; American College of Neuropsychophar-macology, Scottsdale AZ, 2008; University of Tehran, School of Medicine, 2008; Autism One Conference, Chicago, 2009. Funding Information: All experimental procedures were approved by the Institutional Review Board of the University of Minnesota School of Medicine. Postmortem blocks of BA40, BA9, and cerebella (lobar origin unknown) were obtained from the Autism Research Foundation and various brain banks (NICHD Brain and Tissue Bank for Developmental Disorders; TARF; the Harvard Brain Tissue Resource Center, which is supported in part by PHS grant number R24 MH068855; the Brain Endowment Bank, which is funded in part by the National Parkinson Foundation, Inc., Miami, Florida; and the Autism Tissue Program). The tissue",

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AU - Folsom, Timothy D.

AU - Rooney, Robert J.

AU - Patel, Diven H.

AU - Thuras, Paul D.

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