Mu opioid receptor from the zebrafish exhibits functional characteristics as those of mammalian mu opioid receptor

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The functional characterization of the mu opioid receptor from the zebrafish (zfMOR) is reported here. After transfection in HEK-293 cell line, using both peptidergic and nonpeptidergic opioid ligands in the competition and saturation-binding experiments, in addition to the functional assays of 35S-GTPγS-binding assays and intracellular 3′-5′- cyclic adenosine monophosphate (cAMP) level determinations, we demonstrate that zfMOR exhibits a pharmacological profile similar to that of the mammalian MOR. Besides, the internalization process of zfMOR after opiate agonist treatment (morphine, DAMGO, etorphine) resembles the pattern observed for its mammalian counterpart. These similarities suggest that the zebrafish is a good model for the study of the opioid effects in development.

Original languageEnglish (US)
Pages (from-to)259-268
Number of pages10
JournalZebrafish
Volume6
Issue number3
DOIs
StatePublished - Sep 1 2009

Fingerprint

Dive into the research topics of 'Mu opioid receptor from the zebrafish exhibits functional characteristics as those of mammalian mu opioid receptor'. Together they form a unique fingerprint.

Cite this