TY - JOUR
T1 - Multi-probe RPA template sets to study RNA modulation and transcriptional control of BH3-only members of the Bcl-2 family
AU - Wang, Dehua
AU - McDonnell, Maureen A.
AU - Kelekar, Ameeta
N1 - Funding Information:
This study was supported by a grant (3051-9227-01) from the Minnesota Medical Foundation.
PY - 2005
Y1 - 2005
N2 - The Bcl-2 family of apoptotic regulators comprises both pro- and anti-apoptotic proteins. Pro-apoptotic BH3-only members of the Bcl-2 family are subject to stringent control in normal proliferating cells, but can be activated by a variety of mechanisms in response to diverse apoptotic signals. To facilitate the simultaneous identification of BH3-only targets that are regulated at the RNA level, we have designed and assembled multi-probe ribonuclease protection assay templates and demonstrated their ability to detect multiple BH3-only transcripts in a variety of human cell lines and primary tissues. Following this, we have determined the modulation of BH3-only transcripts to a variety of apoptotic stimuli, in selected primary and transformed cell lines. Early experiments, showing that a given cell type upregulates a unique subset of BH3-only RNAs in response to different death-inducing stimuli, indicate that the multi-probe BH3-only RPA template sets could serve as useful diagnostic and analytical tools in cancer research.
AB - The Bcl-2 family of apoptotic regulators comprises both pro- and anti-apoptotic proteins. Pro-apoptotic BH3-only members of the Bcl-2 family are subject to stringent control in normal proliferating cells, but can be activated by a variety of mechanisms in response to diverse apoptotic signals. To facilitate the simultaneous identification of BH3-only targets that are regulated at the RNA level, we have designed and assembled multi-probe ribonuclease protection assay templates and demonstrated their ability to detect multiple BH3-only transcripts in a variety of human cell lines and primary tissues. Following this, we have determined the modulation of BH3-only transcripts to a variety of apoptotic stimuli, in selected primary and transformed cell lines. Early experiments, showing that a given cell type upregulates a unique subset of BH3-only RNAs in response to different death-inducing stimuli, indicate that the multi-probe BH3-only RPA template sets could serve as useful diagnostic and analytical tools in cancer research.
KW - Apoptosis
KW - BH3-only proteins
KW - Bcl-2 family
KW - RNase protection assay
KW - Transcriptional control
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U2 - 10.1016/j.cdp.2004.12.002
DO - 10.1016/j.cdp.2004.12.002
M3 - Article
C2 - 15829380
AN - SCOPUS:17144409080
SN - 0361-090X
VL - 29
SP - 189
EP - 200
JO - Cancer Detection and Prevention
JF - Cancer Detection and Prevention
IS - 2
ER -