Multiple acquisition of magic angle spinning solid-state NMR experiments using one receiver: Application to microcrystalline and membrane protein preparations

T. Gopinath, Gianluigi Veglia

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Abstract Solid-state NMR spectroscopy of proteins is a notoriously low-throughput technique. Relatively low-sensitivity and poor resolution of protein samples require long acquisition times for multidimensional NMR experiments. To speed up data acquisition, we developed a family of experiments called Polarization Optimized Experiments (POE), in which we utilized the orphan spin operators that are discarded in classical multidimensional NMR experiments, recovering them to allow simultaneous acquisition of multiple 2D and 3D experiments, all while using conventional probes with spectrometers equipped with one receiver. POE allow the concatenation of multiple 2D or 3D pulse sequences into a single experiment, thus potentially combining all of the aforementioned advances, boosting the capability of ssNMR spectrometers at least two-fold without the addition of any hardware. In this perspective, we describe the first generation of POE, such as dual acquisition MAS (or DUMAS) methods, and then illustrate the evolution of these experiments into MEIOSIS, a method that enables the simultaneous acquisition of multiple 2D and 3D spectra. Using these new pulse schemes for the solid-state NMR investigation of biopolymers makes it possible to obtain sequential resonance assignments, as well as distance restraints, in about half the experimental time. While designed for acquisition of heteronuclei, these new experiments can be easily implemented for proton detection and coupled with other recent advancements, such as dynamic nuclear polarization (DNP), to improve signal to noise. Finally, we illustrate the application of these methods to microcrystalline protein preparations as well as single and multi-span membrane proteins reconstituted in lipid membranes.

Original languageEnglish (US)
Article number5584
Pages (from-to)143-153
Number of pages11
JournalJournal of Magnetic Resonance
Volume253
DOIs
StatePublished - Apr 2015

Bibliographical note

Funding Information:
This work is supported by the National Institute of Health (GM 64742 and GM 72701). The NMR experiments were carried out at the Minnesota NMR Center.

Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.

Keywords

  • DUMAS
  • MEIOSIS
  • Magic angle spinning
  • Membrane proteins
  • Microcrystalline proteins
  • Multiple acquisition
  • Polarization optimized experiments
  • Solid-state NMR

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