Multiplexed aberration measurement for deep tissue imaging in vivo

Chen Wang; Rui Liu; Daniel E. Milkie; Wenzhi Sun; Zhongchao Tan; Aaron Kerlin; Tsai Wen Chen; Douglas S. Kim; Na Ji

doi:10.1038/nmeth.3068

Multiplexed aberration measurement for deep tissue imaging in vivo

Chen Wang, Rui Liu, Daniel E. Milkie, Wenzhi Sun, Zhongchao Tan, Aaron Kerlin, Tsai Wen Chen, Douglas S. Kim, Na Ji

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111 Scopus citations

Abstract

We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.

Original language	English (US)
Pages (from-to)	1037-1040
Number of pages	4
Journal	Nature Methods
Volume	11
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.3068
State	Published - Jan 1 2014
Externally published	Yes

Bibliographical note

Funding Information:
We thank our colleagues at Janelia Farm Research Campus, Howard Hughes Medical Institute: E. Betzig for helpful discussions; H. Dana, B. MacLennan, G. Ranganathan, K. Smith for help with mice samples; and P. Keller and M. Ahrens for providing zebrafish samples. We thank C. Fang-Yen for advice on C. elegans samples. This work was supported by Howard Hughes Medical Institute.

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AU - Kerlin, Aaron

AU - Chen, Tsai Wen

AU - Kim, Douglas S.

AU - Ji, Na

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Multiplexed aberration measurement for deep tissue imaging in vivo

Abstract

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