Multivalent glycocluster design through directed evolution

Iain S. MacPherson; J. Sebastian Temme; Sevan Habeshian; Krzysztof Felczak; Krzysztof Pankiewicz; Lizbeth Hedstrom; Isaac J. Krauss

doi:10.1002/anie.201105555

Multivalent glycocluster design through directed evolution

Iain S. MacPherson, J. Sebastian Temme, Sevan Habeshian, Krzysztof Felczak, Krzysztof Pankiewicz, Lizbeth Hedstrom, Isaac J. Krauss

Center for Drug Design

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Selection with modified aptamers: A method is described for design of glycocluster ligands by directed evolution. Glycan azides are attached to a library of alkyne-containing DNA sequences. DNA sequences which cluster the glycans best for multivalent binding to the target are selected and amplified. This method has been applied to the development of high-mannose clusters which bind HIV-neutralizing antibody 2G12.

Original language	English (US)
Pages (from-to)	11238-11242
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	47
DOIs	https://doi.org/10.1002/anie.201105555
State	Published - Nov 18 2011

Keywords

carbohydrates
cluster effect
directed evolution
glycoconjugates
multivalency

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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AU - Habeshian, Sevan

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AU - Pankiewicz, Krzysztof

AU - Hedstrom, Lizbeth

AU - Krauss, Isaac J.

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KW - multivalency

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Multivalent glycocluster design through directed evolution

Abstract

Keywords

UN SDGs

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