Abstract
The acetogenin murihexocin C was isolated from the leaves of Annona squamosa Linn. The structure was assigned based on 1H and 13C NMR and HR-ESI-TOF-MS. This is the first report of the compound from this plant. Murihexocin C exhibited significant cytotoxicity to human colon carcinoma Col 2 cells (IC 50 0.50 μg mL -1 or 0.79 μM) and normal Chinese hamster ovarian cells AA8 cells (IC 50 0.78 μg mL -1 or 1.24 μM). Murihexocin C (50 μg mL -1) induced extensive apoptotic cell death in Col 2 cells at 12 h as demonstrated by the TUNEL assay. Activation of the apoptotic effector caspase 3 was observed and a Bcl-2-like protein was noted to be down regulated.
Original language | English (US) |
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Pages (from-to) | 122-132 |
Number of pages | 11 |
Journal | Philippine Agricultural Scientist |
Volume | 92 |
Issue number | 2 |
State | Published - Jun 2009 |
Externally published | Yes |
Keywords
- Anticancer
- Apoptosis
- Cell culture
- Chromatography
- Colon cancer
- Cytotoxicity
- Natural products