Muscle activity and aging affect myosin structural distribution and force generation in rat fibers

Dawn A. Lowe, Gordon L. Warren, Le Ann M. Snow, La Dora V. Thompson, David D. Thomas

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The purpose of this study was to determine whether increased muscle activity could reverse myosin structural alterations that occur in aged rat muscle and whether those alterations could be induced in young rat muscle by decreased activity. Semimembranosus muscle activity was increased by electrical stimulation (200-ms trains, 154 Hz, 5 V) through a nerve cuff on the tibial branch of the ischiatic nerve. The protocol consisted of 5 sets of 6-10 maximal isometric contractions performed twice per week for 4 or 8-10 wk. Decreased muscle activity was induced by denervation of the semimembranosus muscle for 2 or 4 wk. Semi-membranosus fibers were then studied for Ca 2+-activated force generation. Fibers were also spin labeled on the myosin catalytic domain and studied using electron paramagnetic resonance (EPR) spectroscopy to assess myosin structural distribution. Increased muscle activity for 4 and 8-10 wk in ∼32-mo-old rats resulted in -16 and +4% changes in specific tension, respectively (P < 0.01). EPR spectra showed that the fraction of myosin heads in the strong-binding structural state during contraction was reduced at 4 wk (0.241 ± 0.020 vs. 0.269 ± 0.018, P = 0.046) but returned to normal by 8-10 wk (P = 0.67). Decreased muscle activity for 2 and 4 wk in ∼9-mo-old rats resulted in 23 and 34% reductions, respectively, in specific tension; EPR spectra showed 16 and 35% decreases in strong-binding myosin (P < 0.01). These data support the hypothesis that changes in muscle activity affect muscle strength, at least in part through alterations in myosin structure and function.

Original languageEnglish (US)
Pages (from-to)498-506
Number of pages9
JournalJournal of applied physiology
Volume96
Issue number2
DOIs
StatePublished - Feb 2004

Keywords

  • Denervation
  • Electron paramagnetic resonance spectroscopy
  • Permeabilized fiber
  • Specific tension

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