Mycobacterial interspersed repetitive unit typing in mycobacterium tuberculosis isolates from Sichuan province in China

Jian hua Guo, Wen liang Xiang, Geng Zhang, Tao Luo, Ning Xie, Zhi rong Yang, Qun Sun

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Background & objectives: Emergence and spread of drug resistant Mycobacterium tuberculosis is a serious threat to tuberculosis (TB) control programme. Therefore, the objective of this study was to genotype drug-resistant M. tuberculosis strains isolated from patients in Sichuan, China, using Mycobacterial Interspersed Repetitive Units (MIRU) for epidemiological analysis. Methods: Drug-resistance testing of M. tuberculosis isolates from pulmonary TB patients was confirmed by proportion method. Twelve MIRU loci were analyzed on 80 drug-resistant and 9 susceptible isolates by polymerase chain reaction and agarose gel electrophoresis. Hunter-Gaston discriminatory index (HGI) values were determined for each 12 MIRU loci for the evaluation of their discrimination power. Results: Among 12 MIRU loci examined, polymorphic bands could be generated on 11 loci. Sixty five isolates had distinct MIRU patterns, while other 24 belonged to 8 clusters and resistant to at least one anti-TB drug tested. The association between the MIRU patterns and the mutation patterns of drug-resistance relevant target genes was not significant among the drug-resistant isolates. Interpretation & conclusions: The results showed that with a satisfactory discrimination power exhibited, the 12 loci based MIRU typing could be a valuable tool for epidemiological studies in M. tuberculosis isolates from Sichuan.

Original languageEnglish (US)
Pages (from-to)362-368
Number of pages7
JournalIndian Journal of Medical Research
Volume134
Issue number9
StatePublished - Sep 2011
Externally publishedYes

Keywords

  • Drug resistance
  • Genotyping
  • Miru
  • Mycobacterium tuberculosis
  • Sichuan

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