Mycolicibacterium nivoides sp. Nov isolated from a peat bog

John L. Dahl, Wayne Gatlin, Phuong M. Tran, Cody S Sheik

Research output: Contribution to journalArticlepeer-review

Abstract

A fast-growing, non-chromogenic, acid-fast-staining bacterium (DL90T) was isolated from a peat bog in northern Minnesota. On the basis of 16S rRNA gene sequence similarity (99.8 % identity with Mycolicibacterium septicum and 98 % with Mycolicibac-terium peregrinum) and chemotaxonomic data (fatty acid content), strain DL90T represents a member of the genus Mycolici-bacterium. Physiological tests (growth curves, biofilm formation, antibiotic sensitivity, colony morphologies and heat tolerance) and biochemical analysis (arylsulfatase activity and fatty acid profiles) distinguish DL90T from its closest relative M. septicum. Phylogenomic reconstruction of the ‘Fortuitium–Vaccae’ clade, digital DNA–DNA hybridization (DDH) values of 61 %, and average nucleotide identity (ANI) values of approximately 95 % indicate that DL90T is likely to be diverged from M. septicum. Thus, we propose that DL90T represents a novel species, given the name Mycolicibacterium nivoides with the type strain being isolate DL90T (=JCM 32796T=NCCB 100660T).

Original languageEnglish (US)
Article number004438
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume71
Issue number3
DOIs
StatePublished - 2021

Bibliographical note

Funding Information:
This research was supported by internal Grant-In-AID funds from the University of Minnesota.

Publisher Copyright:
© 2021 The Authors.

Keywords

  • Mycolicibacterium
  • Mycolicibacterium nivoides
  • Sphagnum peat bog

PubMed: MeSH publication types

  • Journal Article

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