N-methylnitrosourea-induced carcinoma as a model for laryngeal carcinogenesis

Emiro Caicedo-Granados; Arthur R. Galbraith; Monika G. Schachern; Donna E. Hartle; Lee W. Wattenberg; Beverly R. Wuertz; Suzanne Keel; Bevan Yueh; Frank G. Ondrey

doi:10.1002/hed.23536

N-methylnitrosourea-induced carcinoma as a model for laryngeal carcinogenesis

Emiro Caicedo-Granados, Arthur R. Galbraith, Monika G. Schachern, Donna E. Hartle, Lee W. Wattenberg, Beverly R. Wuertz, Suzanne Keel, Bevan Yueh, Frank G. Ondrey

Otolaryngology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Background. Preclinical animal models to study laryngeal cancer are nonexistent. The purpose of this study was to describe a novel mice laryngeal cancer model.

Methods. A total of 18 six-week-old A/J mice were used. Animals underwent microdirect laryngoscopy, superficial larynx scratching, and instillation of N-methyl-N-nitrosourea (MNU) at 2 different concentrations (15 lL and 30 lL) or dimethyl sulfoxide (DMSO) to the control group directly to the larynx. Mice received a total of 5 instillations of MNU or DMSO at 1-week intervals. Mice were euthanized at 20 and 30 weeks after the last intervention and laryngeal histology was analyzed.

Results. Laryngeal instillation of MNU caused a 60% cancer conversion in the study group.

Conclusion. Our findings demonstrate the feasibility of developing a murine laryngeal carcinogenesis model using direct topical instillation of MNU. This is the first murine model of laryngeal cancer and has great potential for evaluating new agents for chemoprevention and treatment for laryngeal carcinoma.

Original language	English (US)
Pages (from-to)	1802-1806
Number of pages	5
Journal	Head and Neck
Volume	36
Issue number	12
DOIs	https://doi.org/10.1002/hed.23536
State	Published - Dec 1 2014

Bibliographical note

Publisher Copyright:
© 2014 Wiley Periodicals, Inc.

Keywords

Animal model
Carcinogenesis
Carcinoma
Dysplasia
Larynx

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abstract = "Background. Preclinical animal models to study laryngeal cancer are nonexistent. The purpose of this study was to describe a novel mice laryngeal cancer model.Methods. A total of 18 six-week-old A/J mice were used. Animals underwent microdirect laryngoscopy, superficial larynx scratching, and instillation of N-methyl-N-nitrosourea (MNU) at 2 different concentrations (15 lL and 30 lL) or dimethyl sulfoxide (DMSO) to the control group directly to the larynx. Mice received a total of 5 instillations of MNU or DMSO at 1-week intervals. Mice were euthanized at 20 and 30 weeks after the last intervention and laryngeal histology was analyzed.Results. Laryngeal instillation of MNU caused a 60% cancer conversion in the study group.Conclusion. Our findings demonstrate the feasibility of developing a murine laryngeal carcinogenesis model using direct topical instillation of MNU. This is the first murine model of laryngeal cancer and has great potential for evaluating new agents for chemoprevention and treatment for laryngeal carcinoma.",

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AU - Galbraith, Arthur R.

AU - Schachern, Monika G.

AU - Hartle, Donna E.

AU - Wattenberg, Lee W.

AU - Wuertz, Beverly R.

AU - Keel, Suzanne

AU - Yueh, Bevan

AU - Ondrey, Frank G.

PY - 2014/12/1

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N2 - Background. Preclinical animal models to study laryngeal cancer are nonexistent. The purpose of this study was to describe a novel mice laryngeal cancer model.Methods. A total of 18 six-week-old A/J mice were used. Animals underwent microdirect laryngoscopy, superficial larynx scratching, and instillation of N-methyl-N-nitrosourea (MNU) at 2 different concentrations (15 lL and 30 lL) or dimethyl sulfoxide (DMSO) to the control group directly to the larynx. Mice received a total of 5 instillations of MNU or DMSO at 1-week intervals. Mice were euthanized at 20 and 30 weeks after the last intervention and laryngeal histology was analyzed.Results. Laryngeal instillation of MNU caused a 60% cancer conversion in the study group.Conclusion. Our findings demonstrate the feasibility of developing a murine laryngeal carcinogenesis model using direct topical instillation of MNU. This is the first murine model of laryngeal cancer and has great potential for evaluating new agents for chemoprevention and treatment for laryngeal carcinoma.

AB - Background. Preclinical animal models to study laryngeal cancer are nonexistent. The purpose of this study was to describe a novel mice laryngeal cancer model.Methods. A total of 18 six-week-old A/J mice were used. Animals underwent microdirect laryngoscopy, superficial larynx scratching, and instillation of N-methyl-N-nitrosourea (MNU) at 2 different concentrations (15 lL and 30 lL) or dimethyl sulfoxide (DMSO) to the control group directly to the larynx. Mice received a total of 5 instillations of MNU or DMSO at 1-week intervals. Mice were euthanized at 20 and 30 weeks after the last intervention and laryngeal histology was analyzed.Results. Laryngeal instillation of MNU caused a 60% cancer conversion in the study group.Conclusion. Our findings demonstrate the feasibility of developing a murine laryngeal carcinogenesis model using direct topical instillation of MNU. This is the first murine model of laryngeal cancer and has great potential for evaluating new agents for chemoprevention and treatment for laryngeal carcinoma.

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KW - Carcinogenesis

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KW - Dysplasia

KW - Larynx

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N-methylnitrosourea-induced carcinoma as a model for laryngeal carcinogenesis

Abstract

Bibliographical note

Keywords

UN SDGs

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