A fundamental task in systems biology is to quantify the contributions of the systems’ parts and their interactions. Here I describe a powerful concept and tool for this purpose: Network reconstitution. Genotypes of an organism that represent all possible combinations of the subnetworks in question will be quantitatively phenotyped. The quantitative phenotype data is analyzed using an R script to obtain estimates for single subnetwork contributions and their interactions.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||9|
|State||Published - 2017|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
I thank Rachel Hillmer for critical reading of the manuscript. This work was supported by grants from National Science Foundation, MCB-0918908, IOS-1121425, and MCB-1518058.
© Springer Science+Business Media LLC 2017.
- Combinatorial genotypes
- Network reconstitution
- Quantitative phenotype
- Signaling allocation
- Subnetwork interactions