Neurite outgrowth is dependent on the association of c-Src and lipid rafts

Hui Zhao; Xiaoding Cao; Gencheng Wu; Horace H. Loh; Ping Yee Law

doi:10.1007/s11064-009-0016-7

Neurite outgrowth is dependent on the association of c-Src and lipid rafts

Hui Zhao, Xiaoding Cao, Gencheng Wu, Horace H. Loh, Ping Yee Law

Pharmacology

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14 Scopus citations

Abstract

Regulation of neurite outgrowth is an important aspect not only for proper development of the nervous system but also for tissue regeneration after nerve injury and the treatment of neuropathological conditions. Here, we report that neurite outgrowth in cortical neuron and neuro 2A (N2A) cell was dependent on intact lipid rafts, as well as the enhanced localization of c-Src in the lipid rafts. Src inhibition or lipid rafts disruption could specifically block c-Src phosphorylation profile, pY416 Src increase and pY529 Src decrease, they also resulted in pY529 Src and c-terminal Src kinase (Csk) partition out of lipid rafts. Thus, we concluded that c-Src signal cascades within the lipid rafts is crucial for efficient neurite outgrowth.

Original language	English (US)
Pages (from-to)	2197-2205
Number of pages	9
Journal	Neurochemical Research
Volume	34
Issue number	12
DOIs	https://doi.org/10.1007/s11064-009-0016-7
State	Published - Dec 2009

Keywords

Lipid rafts
Neurite outgrowth, c-Src
Src phosphorylation

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abstract = "Regulation of neurite outgrowth is an important aspect not only for proper development of the nervous system but also for tissue regeneration after nerve injury and the treatment of neuropathological conditions. Here, we report that neurite outgrowth in cortical neuron and neuro 2A (N2A) cell was dependent on intact lipid rafts, as well as the enhanced localization of c-Src in the lipid rafts. Src inhibition or lipid rafts disruption could specifically block c-Src phosphorylation profile, pY416 Src increase and pY529 Src decrease, they also resulted in pY529 Src and c-terminal Src kinase (Csk) partition out of lipid rafts. Thus, we concluded that c-Src signal cascades within the lipid rafts is crucial for efficient neurite outgrowth.",

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AU - Cao, Xiaoding

AU - Wu, Gencheng

AU - Loh, Horace H.

AU - Law, Ping Yee

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Neurite outgrowth is dependent on the association of c-Src and lipid rafts

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