Neuroblastoma X glioma NG108-15 hybrid cells cultured in a chemically defined medium within 3 cell passages, exhibited viability, growth rate and morphology similar to those of cells grown in medium supplemented with 5% fetal calf serum. Hybrid cells cultured in the chemically defined medium within these periods of time also did not exhibit a difference in basal adenylate cyclase activity nor in the enzymatic activities stimulated by adenosine, forskolin, NaF, GppNHp or Mn2+. Furthermore, opiate receptor density in chemically defined medium cultured cells remained identical to that in cells cultured in 5% fetal calf serum. The acute and chronic effects of opiates on adenylate cyclase were similar for cells grown under either set of conditions.
Bibliographical noteFunding Information:
The present research is supported in part by NIDA Grant DA 000564 and Army Contract DAMD17-84-C-4019. H.H.L. is a recipient of National Institute of Drug Abuse Career Award K02-DA-70554.
- adenylate cyclase activity
- neuroblastoma X glioma NG108-15 hybrid cell
- opiate regulation
- serum-free chemically defined medium