Neuroblastoma X glioma NG108-15 hybrid cells cultured in a serum-free chemically defined medium: effects on acute and chronic opiate regulation of adenylate cyclase activity

Michael T. Griffin, Ping Yee Law, Horace H. Loh

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Neuroblastoma X glioma NG108-15 hybrid cells cultured in a chemically defined medium within 3 cell passages, exhibited viability, growth rate and morphology similar to those of cells grown in medium supplemented with 5% fetal calf serum. Hybrid cells cultured in the chemically defined medium within these periods of time also did not exhibit a difference in basal adenylate cyclase activity nor in the enzymatic activities stimulated by adenosine, forskolin, NaF, GppNHp or Mn2+. Furthermore, opiate receptor density in chemically defined medium cultured cells remained identical to that in cells cultured in 5% fetal calf serum. The acute and chronic effects of opiates on adenylate cyclase were similar for cells grown under either set of conditions.

Original languageEnglish (US)
Pages (from-to)370-373
Number of pages4
JournalBrain Research
Volume360
Issue number1-2
DOIs
StatePublished - Dec 23 1985

Bibliographical note

Funding Information:
The present research is supported in part by NIDA Grant DA 000564 and Army Contract DAMD17-84-C-4019. H.H.L. is a recipient of National Institute of Drug Abuse Career Award K02-DA-70554.

Keywords

  • adenylate cyclase activity
  • neuroblastoma X glioma NG108-15 hybrid cell
  • opiate regulation
  • serum-free chemically defined medium

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