Jejunal Peyer's patches (JPP) are innervated sites of immune induction and enteropathogen infection. We investigated the role of enteric nerves in modulating pathogen entry into porcine JPP. Presumptive norepinephrine (NE)-containing nerve fibers were localized in JPP domes and follicle-associated villi by secondary immunofluorescence histochemistry. NE or the neuronal conduction blocker saxitoxin increased intracellular internalization of pathogenic Salmonella choleraesuis and Escherichia coli O157:H7, but not nonpathogenic E. coli, into isolated JPP mucosa. NE action was prevented by the α-adrenergic antagonist phentolamine. Withdrawal of enteric neural activity or NE administration appears to modulate JPP interactions with pathogenic bacteria.
Bibliographical noteFunding Information:
This work was supported in part by National Institutes of Health grants R01 DA-10200, R01 AI-44918, and T32 DA-007239.
- Enteric nervous system
- Enterohemorrhagic Escherichia coli
- Mucosal immunity
- Salmonella enterica serovar choleraesuis
- Sympathetic nerves