Neuromodulation of enteropathogen internalization in Peyer's patches from porcine jejunum

Benedict T. Green; Mark Lyte; Anjali Kulkarni-Narla; David R. Brown

doi:10.1016/S0165-5728(03)00225-X

Neuromodulation of enteropathogen internalization in Peyer's patches from porcine jejunum

Benedict T. Green, Mark Lyte, Anjali Kulkarni-Narla, David R. Brown

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81 Scopus citations

Abstract

Jejunal Peyer's patches (JPP) are innervated sites of immune induction and enteropathogen infection. We investigated the role of enteric nerves in modulating pathogen entry into porcine JPP. Presumptive norepinephrine (NE)-containing nerve fibers were localized in JPP domes and follicle-associated villi by secondary immunofluorescence histochemistry. NE or the neuronal conduction blocker saxitoxin increased intracellular internalization of pathogenic Salmonella choleraesuis and Escherichia coli O157:H7, but not nonpathogenic E. coli, into isolated JPP mucosa. NE action was prevented by the α-adrenergic antagonist phentolamine. Withdrawal of enteric neural activity or NE administration appears to modulate JPP interactions with pathogenic bacteria.

Original language	English (US)
Pages (from-to)	74-82
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	141
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-5728(03)00225-X
State	Published - Aug 2003

Bibliographical note

Funding Information:
This work was supported in part by National Institutes of Health grants R01 DA-10200, R01 AI-44918, and T32 DA-007239.

Keywords

Enteric nervous system
Enterohemorrhagic Escherichia coli
Mucosal immunity
Salmonella enterica serovar choleraesuis
Sympathetic nerves

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title = "Neuromodulation of enteropathogen internalization in Peyer's patches from porcine jejunum",

abstract = "Jejunal Peyer's patches (JPP) are innervated sites of immune induction and enteropathogen infection. We investigated the role of enteric nerves in modulating pathogen entry into porcine JPP. Presumptive norepinephrine (NE)-containing nerve fibers were localized in JPP domes and follicle-associated villi by secondary immunofluorescence histochemistry. NE or the neuronal conduction blocker saxitoxin increased intracellular internalization of pathogenic Salmonella choleraesuis and Escherichia coli O157:H7, but not nonpathogenic E. coli, into isolated JPP mucosa. NE action was prevented by the α-adrenergic antagonist phentolamine. Withdrawal of enteric neural activity or NE administration appears to modulate JPP interactions with pathogenic bacteria.",

keywords = "Enteric nervous system, Enterohemorrhagic Escherichia coli, Mucosal immunity, Salmonella enterica serovar choleraesuis, Sympathetic nerves",

author = "Green, {Benedict T.} and Mark Lyte and Anjali Kulkarni-Narla and Brown, {David R.}",

note = "Funding Information: This work was supported in part by National Institutes of Health grants R01 DA-10200, R01 AI-44918, and T32 DA-007239. ",

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AU - Green, Benedict T.

AU - Lyte, Mark

AU - Kulkarni-Narla, Anjali

AU - Brown, David R.

N1 - Funding Information: This work was supported in part by National Institutes of Health grants R01 DA-10200, R01 AI-44918, and T32 DA-007239.

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N2 - Jejunal Peyer's patches (JPP) are innervated sites of immune induction and enteropathogen infection. We investigated the role of enteric nerves in modulating pathogen entry into porcine JPP. Presumptive norepinephrine (NE)-containing nerve fibers were localized in JPP domes and follicle-associated villi by secondary immunofluorescence histochemistry. NE or the neuronal conduction blocker saxitoxin increased intracellular internalization of pathogenic Salmonella choleraesuis and Escherichia coli O157:H7, but not nonpathogenic E. coli, into isolated JPP mucosa. NE action was prevented by the α-adrenergic antagonist phentolamine. Withdrawal of enteric neural activity or NE administration appears to modulate JPP interactions with pathogenic bacteria.

AB - Jejunal Peyer's patches (JPP) are innervated sites of immune induction and enteropathogen infection. We investigated the role of enteric nerves in modulating pathogen entry into porcine JPP. Presumptive norepinephrine (NE)-containing nerve fibers were localized in JPP domes and follicle-associated villi by secondary immunofluorescence histochemistry. NE or the neuronal conduction blocker saxitoxin increased intracellular internalization of pathogenic Salmonella choleraesuis and Escherichia coli O157:H7, but not nonpathogenic E. coli, into isolated JPP mucosa. NE action was prevented by the α-adrenergic antagonist phentolamine. Withdrawal of enteric neural activity or NE administration appears to modulate JPP interactions with pathogenic bacteria.

KW - Enteric nervous system

KW - Enterohemorrhagic Escherichia coli

KW - Mucosal immunity

KW - Salmonella enterica serovar choleraesuis

KW - Sympathetic nerves

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ER -

Neuromodulation of enteropathogen internalization in Peyer's patches from porcine jejunum

Abstract

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Keywords

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