Neurotensin and cholecystokinin contract gallbladder circular muscle in chickens

T. F. Degolier, D. R. Brown, G. E. Duke, M. M. Palmer, J. R. Swenson, R. E. Carraway

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The contractile effects of neurotensin (NT) and cholecystokinin octapeptide (CCK-8) on isolated circular smooth muscle strips of chicken gallbladder were investigated. The NT (0.25-300 nM) produced concentration-dependent contractions on smooth muscle with an EC50 of 8.5 nM (95% confidence limits = 5.3-13.6 nM). In comparison, CCK-8 produced concentration-dependent contractions with an EC50 of 13 nM (95% confidence limits of 9-20 nM). There were no statistical differences in contractile responses when comparing NT and CCK-8 at equimolar concentrations. The NT appears to act directly on smooth muscle tissue in the chicken; the contractile responses were not blocked by 10 μM atropine or tetrodotoxin.A portion of the activity is mediated by extracellular calcium as 100 nM nifedipine inhibited 30% of peptide-induced muscle tension. The NT receptor (NTR) type 1 antagonist SR 48692 (0.1 μM) did not significantly reduce NT potency. The contractile effects of CCK-8 remained unaltered in tissues pretreated with atropine, TTX, or nifedipine. The CCK-A antagonist lorglumide, at a concentration of 1 μM, reduced the contractile potency of CCK-8 by one-half. Avian receptors for NT and CCK may differ pharmacologically from their mammalian counterparts, but their contractile actions on the gallbladder resulting in increased biliary output by flow are further evidence of their role in the postprandial regulation of lipid digestion in chickens.

Original languageEnglish (US)
Pages (from-to)2156-2162
Number of pages7
JournalPoultry science
Volume92
Issue number8
DOIs
StatePublished - Aug 1 2013

Keywords

  • Chicken
  • Cholecystokinin
  • Contraction
  • Gallbladder
  • Neurotensin

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