TY - JOUR
T1 - Neutrophil involvement in cross-priming CD8+ T cell responses to bacterial antigens
AU - Tvinnereim, Amy R.
AU - Hamilton, Sara E.
AU - Harty, John T.
PY - 2004/8/1
Y1 - 2004/8/1
N2 - Substantial CD8+ T cell responses are generated after infection of mice with recombinant Listeria monocytogenes strains expressing a model epitope (lymphocytic choriomeningitis virus NP118-126) in secreted and nonsecreted forms. L. monocytogenes gains access to the cytosol of infected cells, where secreted Ags can be accessed by the endogenous MHC class I presentation pathway. However, the route of presentation of the nonsecreted Ag in vivo remains undefined. In this study we show that neutrophil-enriched peritoneal exudate cells from L. monocytogenes-infected mice can serve as substrates for in vitro cross-presentation of both nonsecreted and secreted Ag by dendritic cells as well as for in vivo cross-priming of CD8+ T cells. In addition, specific neutrophil depletion in vivo by low dose treatment with either of two Ly6G-specific mAb substantially decreased the relative CD8+ T cell response against the nonsecreted, but not the secreted, Ag compared with control Ab-treated mice. Thus, neutrophils not only provide rapid innate defense against infection, bot also contribute to shaping the specificity and breadth of the CD8+ T cell response. In addition, cross-presentation of bacterial Ags from neutrophils may explain how CD8 + T cell responses are generated against Ags from extracellular bacterial pathogens.
AB - Substantial CD8+ T cell responses are generated after infection of mice with recombinant Listeria monocytogenes strains expressing a model epitope (lymphocytic choriomeningitis virus NP118-126) in secreted and nonsecreted forms. L. monocytogenes gains access to the cytosol of infected cells, where secreted Ags can be accessed by the endogenous MHC class I presentation pathway. However, the route of presentation of the nonsecreted Ag in vivo remains undefined. In this study we show that neutrophil-enriched peritoneal exudate cells from L. monocytogenes-infected mice can serve as substrates for in vitro cross-presentation of both nonsecreted and secreted Ag by dendritic cells as well as for in vivo cross-priming of CD8+ T cells. In addition, specific neutrophil depletion in vivo by low dose treatment with either of two Ly6G-specific mAb substantially decreased the relative CD8+ T cell response against the nonsecreted, but not the secreted, Ag compared with control Ab-treated mice. Thus, neutrophils not only provide rapid innate defense against infection, bot also contribute to shaping the specificity and breadth of the CD8+ T cell response. In addition, cross-presentation of bacterial Ags from neutrophils may explain how CD8 + T cell responses are generated against Ags from extracellular bacterial pathogens.
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U2 - 10.4049/jimmunol.173.3.1994
DO - 10.4049/jimmunol.173.3.1994
M3 - Article
C2 - 15265934
AN - SCOPUS:3242798012
SN - 0022-1767
VL - 173
SP - 1994
EP - 2002
JO - Journal of Immunology
JF - Journal of Immunology
IS - 3
ER -