Nitrogen recovery from fertilizer and use efficiency response to Bradyrhizobium sp. and Azospirillum brasilense combined with N rates in cowpea-wheat crop sequence

Fernando Shintate Galindo; Edson Cabral da Silva; Paulo Humberto Pagliari; Guilherme Carlos Fernandes; Willian Lima Rodrigues; Antônio Leonardo Campos Biagini; Eduardo Bianchi Baratella; Castro Alves da Silva Júnior; Mário João Moretti Neto; Vinicius Martins Silva; Takashi Muraoka; Marcelo Carvalho Minhoto Teixeira Filho

doi:10.1016/j.apsoil.2020.103764

Nitrogen recovery from fertilizer and use efficiency response to Bradyrhizobium sp. and Azospirillum brasilense combined with N rates in cowpea-wheat crop sequence

Fernando Shintate Galindo, Edson Cabral da Silva, Paulo Humberto Pagliari, Guilherme Carlos Fernandes, Willian Lima Rodrigues, Antônio Leonardo Campos Biagini, Eduardo Bianchi Baratella, Castro Alves da Silva Júnior, Mário João Moretti Neto, Vinicius Martins Silva, Takashi Muraoka, Marcelo Carvalho Minhoto Teixeira Filho

Southwest Research and Outreach Center

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18 Scopus citations

Abstract

A better understanding of nitrogen (N) behavior and agronomic practices is needed to improve biological nitrogen fixation (BNF) and N use efficiency under field conditions. This study aims on investigating the potential for the use of biological agents that can fix atmospheric N and are related to multiple mechanisms benefits to improve cowpea (Vigna unguiculata (L.) Walp.) development and productivity, leading to a positive residual effect on wheat (Triticum aestivum L.) development. The study was set up under a no-till system in a Rhodic Haplustox, in a randomized complete block design, with four replicates. Treatments were tested in a full factorial design and included: i) three types of inoculation (without inoculation – control; Bradyrhizobium sp. inoculation (strains SEMIA 6462 and SEMIA 6463) – the conventional inoculation of cowpea; and Bradyrhizobium sp. combined with A. brasilense (strains Ab-V5 and Ab-V6) – the co-inoculation); and ii) five N rates (0 to 160 kg N ha⁻¹) applied as urea-¹⁵N isotope. The residual effects of inoculations and N application rates applied during the cowpea growing season were evaluated on wheat (successor crop). Co-inoculation was found to increase cowpea grain yield by 40.5% and 14.1% compared to the control and Bradyrhizobium sp. inoculation when N was omitted and up to 37.6% and 50.8% when N was applied. Co-inoculation increased N use efficiency, N recovery and N accumulation on cowpea, leading to improved crop growth. The co-inoculation also provided a positive residual effect on wheat resulting in increased plant N accumulation, crop development, and greater wheat grain yield compared to the control (increase of 7.8%) and Bradyrhizobium sp. inoculated plants (increase of 5.8%). Co-inoculation was found to increase N use efficiency on cowpea by 216.5% and 35.5% and on wheat by 159.3% and 29.5% compared to control and Bradyrhizobium sp. inoculation, respectively. The fertilizer N recovery was, on average, 22.2% by cowpea, while fertilizer N recovery by wheat was <2.3%. The percentage of N accumulated on cowpea was on average 30.8%, while the residual cowpea N contribution to wheat provided by co-inoculation was 18.6%. This study showed positive improvements in cowpea-wheat growth production parameters and N management as a result of co-inoculation, indicating that there is an opportunity to reduce N fertilizer input with the combined use of biological agents that promote plant growth and chemical fertilizers.

Original language	English (US)
Article number	103764
Journal	Applied Soil Ecology
Volume	157
DOIs	https://doi.org/10.1016/j.apsoil.2020.103764
State	Published - Jan 2021

Bibliographical note

Funding Information:
This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP [grant number 18/08485-7 ] and Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq [grant number 312359/2017-9 ].

Funding Information:
This work was supported by the Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo - FAPESP [grant number 18/08485-7] and Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq [grant number 312359/2017-9].

Publisher Copyright:
© 2020 Elsevier B.V.

Keywords

Biological nitrogen fixation
Efficiency of nitrogen fertilization
Nitrogen-15
Plant growth promoting bacteria
Residual effect of N

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Galindo, F. S., da Silva, E. C., Pagliari, P. H., Fernandes, G. C., Rodrigues, W. L., Biagini, A. L. C., Baratella, E. B., da Silva Júnior, C. A., Moretti Neto, M. J., Silva, V. M., Muraoka, T., & Teixeira Filho, M. C. M. (2021). Nitrogen recovery from fertilizer and use efficiency response to Bradyrhizobium sp. and Azospirillum brasilense combined with N rates in cowpea-wheat crop sequence. Applied Soil Ecology, 157, Article 103764. https://doi.org/10.1016/j.apsoil.2020.103764

Nitrogen recovery from fertilizer and use efficiency response to Bradyrhizobium sp. and Azospirillum brasilense combined with N rates in cowpea-wheat crop sequence. / Galindo, Fernando Shintate; da Silva, Edson Cabral; Pagliari, Paulo Humberto et al.
In: Applied Soil Ecology, Vol. 157, 103764, 01.2021.

Research output: Contribution to journal › Article › peer-review

Galindo, FS, da Silva, EC, Pagliari, PH, Fernandes, GC, Rodrigues, WL, Biagini, ALC, Baratella, EB, da Silva Júnior, CA, Moretti Neto, MJ, Silva, VM, Muraoka, T & Teixeira Filho, MCM 2021, 'Nitrogen recovery from fertilizer and use efficiency response to Bradyrhizobium sp. and Azospirillum brasilense combined with N rates in cowpea-wheat crop sequence', Applied Soil Ecology, vol. 157, 103764. https://doi.org/10.1016/j.apsoil.2020.103764

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abstract = "A better understanding of nitrogen (N) behavior and agronomic practices is needed to improve biological nitrogen fixation (BNF) and N use efficiency under field conditions. This study aims on investigating the potential for the use of biological agents that can fix atmospheric N and are related to multiple mechanisms benefits to improve cowpea (Vigna unguiculata (L.) Walp.) development and productivity, leading to a positive residual effect on wheat (Triticum aestivum L.) development. The study was set up under a no-till system in a Rhodic Haplustox, in a randomized complete block design, with four replicates. Treatments were tested in a full factorial design and included: i) three types of inoculation (without inoculation – control; Bradyrhizobium sp. inoculation (strains SEMIA 6462 and SEMIA 6463) – the conventional inoculation of cowpea; and Bradyrhizobium sp. combined with A. brasilense (strains Ab-V5 and Ab-V6) – the co-inoculation); and ii) five N rates (0 to 160 kg N ha−1) applied as urea-15N isotope. The residual effects of inoculations and N application rates applied during the cowpea growing season were evaluated on wheat (successor crop). Co-inoculation was found to increase cowpea grain yield by 40.5% and 14.1% compared to the control and Bradyrhizobium sp. inoculation when N was omitted and up to 37.6% and 50.8% when N was applied. Co-inoculation increased N use efficiency, N recovery and N accumulation on cowpea, leading to improved crop growth. The co-inoculation also provided a positive residual effect on wheat resulting in increased plant N accumulation, crop development, and greater wheat grain yield compared to the control (increase of 7.8%) and Bradyrhizobium sp. inoculated plants (increase of 5.8%). Co-inoculation was found to increase N use efficiency on cowpea by 216.5% and 35.5% and on wheat by 159.3% and 29.5% compared to control and Bradyrhizobium sp. inoculation, respectively. The fertilizer N recovery was, on average, 22.2% by cowpea, while fertilizer N recovery by wheat was <2.3%. The percentage of N accumulated on cowpea was on average 30.8%, while the residual cowpea N contribution to wheat provided by co-inoculation was 18.6%. This study showed positive improvements in cowpea-wheat growth production parameters and N management as a result of co-inoculation, indicating that there is an opportunity to reduce N fertilizer input with the combined use of biological agents that promote plant growth and chemical fertilizers.",

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note = "Funding Information: This work was supported by the Funda{\c c}{\~a}o de Amparo {\`a} Pesquisa do Estado de S{\~a}o Paulo - FAPESP [grant number 18/08485-7 ] and Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico - CNPq [grant number 312359/2017-9 ]. Funding Information: This work was supported by the Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo - FAPESP [grant number 18/08485-7] and Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq [grant number 312359/2017-9]. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

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AU - Galindo, Fernando Shintate

AU - da Silva, Edson Cabral

AU - Pagliari, Paulo Humberto

AU - Fernandes, Guilherme Carlos

AU - Rodrigues, Willian Lima

AU - Biagini, Antônio Leonardo Campos

AU - Baratella, Eduardo Bianchi

AU - da Silva Júnior, Castro Alves

AU - Moretti Neto, Mário João

AU - Silva, Vinicius Martins

AU - Muraoka, Takashi

AU - Teixeira Filho, Marcelo Carvalho Minhoto

N1 - Funding Information: This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP [grant number 18/08485-7 ] and Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq [grant number 312359/2017-9 ]. Funding Information: This work was supported by the Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo - FAPESP [grant number 18/08485-7] and Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq [grant number 312359/2017-9]. Publisher Copyright: © 2020 Elsevier B.V.

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N2 - A better understanding of nitrogen (N) behavior and agronomic practices is needed to improve biological nitrogen fixation (BNF) and N use efficiency under field conditions. This study aims on investigating the potential for the use of biological agents that can fix atmospheric N and are related to multiple mechanisms benefits to improve cowpea (Vigna unguiculata (L.) Walp.) development and productivity, leading to a positive residual effect on wheat (Triticum aestivum L.) development. The study was set up under a no-till system in a Rhodic Haplustox, in a randomized complete block design, with four replicates. Treatments were tested in a full factorial design and included: i) three types of inoculation (without inoculation – control; Bradyrhizobium sp. inoculation (strains SEMIA 6462 and SEMIA 6463) – the conventional inoculation of cowpea; and Bradyrhizobium sp. combined with A. brasilense (strains Ab-V5 and Ab-V6) – the co-inoculation); and ii) five N rates (0 to 160 kg N ha−1) applied as urea-15N isotope. The residual effects of inoculations and N application rates applied during the cowpea growing season were evaluated on wheat (successor crop). Co-inoculation was found to increase cowpea grain yield by 40.5% and 14.1% compared to the control and Bradyrhizobium sp. inoculation when N was omitted and up to 37.6% and 50.8% when N was applied. Co-inoculation increased N use efficiency, N recovery and N accumulation on cowpea, leading to improved crop growth. The co-inoculation also provided a positive residual effect on wheat resulting in increased plant N accumulation, crop development, and greater wheat grain yield compared to the control (increase of 7.8%) and Bradyrhizobium sp. inoculated plants (increase of 5.8%). Co-inoculation was found to increase N use efficiency on cowpea by 216.5% and 35.5% and on wheat by 159.3% and 29.5% compared to control and Bradyrhizobium sp. inoculation, respectively. The fertilizer N recovery was, on average, 22.2% by cowpea, while fertilizer N recovery by wheat was <2.3%. The percentage of N accumulated on cowpea was on average 30.8%, while the residual cowpea N contribution to wheat provided by co-inoculation was 18.6%. This study showed positive improvements in cowpea-wheat growth production parameters and N management as a result of co-inoculation, indicating that there is an opportunity to reduce N fertilizer input with the combined use of biological agents that promote plant growth and chemical fertilizers.

AB - A better understanding of nitrogen (N) behavior and agronomic practices is needed to improve biological nitrogen fixation (BNF) and N use efficiency under field conditions. This study aims on investigating the potential for the use of biological agents that can fix atmospheric N and are related to multiple mechanisms benefits to improve cowpea (Vigna unguiculata (L.) Walp.) development and productivity, leading to a positive residual effect on wheat (Triticum aestivum L.) development. The study was set up under a no-till system in a Rhodic Haplustox, in a randomized complete block design, with four replicates. Treatments were tested in a full factorial design and included: i) three types of inoculation (without inoculation – control; Bradyrhizobium sp. inoculation (strains SEMIA 6462 and SEMIA 6463) – the conventional inoculation of cowpea; and Bradyrhizobium sp. combined with A. brasilense (strains Ab-V5 and Ab-V6) – the co-inoculation); and ii) five N rates (0 to 160 kg N ha−1) applied as urea-15N isotope. The residual effects of inoculations and N application rates applied during the cowpea growing season were evaluated on wheat (successor crop). Co-inoculation was found to increase cowpea grain yield by 40.5% and 14.1% compared to the control and Bradyrhizobium sp. inoculation when N was omitted and up to 37.6% and 50.8% when N was applied. Co-inoculation increased N use efficiency, N recovery and N accumulation on cowpea, leading to improved crop growth. The co-inoculation also provided a positive residual effect on wheat resulting in increased plant N accumulation, crop development, and greater wheat grain yield compared to the control (increase of 7.8%) and Bradyrhizobium sp. inoculated plants (increase of 5.8%). Co-inoculation was found to increase N use efficiency on cowpea by 216.5% and 35.5% and on wheat by 159.3% and 29.5% compared to control and Bradyrhizobium sp. inoculation, respectively. The fertilizer N recovery was, on average, 22.2% by cowpea, while fertilizer N recovery by wheat was <2.3%. The percentage of N accumulated on cowpea was on average 30.8%, while the residual cowpea N contribution to wheat provided by co-inoculation was 18.6%. This study showed positive improvements in cowpea-wheat growth production parameters and N management as a result of co-inoculation, indicating that there is an opportunity to reduce N fertilizer input with the combined use of biological agents that promote plant growth and chemical fertilizers.

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KW - Efficiency of nitrogen fertilization

KW - Nitrogen-15

KW - Plant growth promoting bacteria

KW - Residual effect of N

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Nitrogen recovery from fertilizer and use efficiency response to Bradyrhizobium sp. and Azospirillum brasilense combined with N rates in cowpea-wheat crop sequence

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