NMR measurement of brain oxidative metabolism in monkeys using 13C-labeled glucose without a 13C radiofrequency channel

Fawzi Boumezbeur, Laurent Besret, Julien Valette, Françoise Vaufrey, Pierre Gilles Henry, Velislav Slavov, Eric Giacomini, Philippe Hantraye, Gilles Bloch, Vincent Lebon

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

We detected glutamate C4 and C3 labeling in the monkey brain during an infusion of [U-13C6]glucose, using a simple 1H PRESS sequence without 13C editing or decoupling. Point-resolved spectroscopy (PRESS) spectra revealed decreases in 12C-bonded protons, and increases in 13C-bonded protons of glutamate. To take full advantage of the simultaneous detection of 12C- and 13C-bonded protons, we implemented a quantitation procedure to properly measure both glutamate C4 and C3 enrichments. This procedure relies on LCModel analysis with a basis set to account for simultaneous signal changes of protons bound to 12C and 13C. Signal changes were mainly attributed to 12C- and 13C-bonded protons of glutamate. As a result, we were able to measure the tricarboxylic acid (TCA) cycle flux in a 3.9 cm3 voxel centered in the monkey brain on a whole-body 3 Tesla system (VTCA = 0.55 ± 0.04 μmol.g-1.min -1 N = 4). This work demonstrates that oxidative metabolism can be quantified in deep structures of the brain on clinical MRI systems, without the need for a 13C radiofrequency (RF) channel.

Original languageEnglish (US)
Pages (from-to)33-40
Number of pages8
JournalMagnetic resonance in medicine
Volume52
Issue number1
DOIs
StatePublished - Jul 1 2004

Keywords

  • Brain
  • LC model
  • Monkey
  • NMR spectroscopy
  • TCA cycle

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