No evidence for structural or functional identity between p24/CD9 and p21/ras

Le Bien Tucker W.; John H. Kersey

doi:10.1016/0145-2126(85)90050-5

No evidence for structural or functional identity between p24/CD9 and p21/ras

Le Bien Tucker W., John H. Kersey

Health Sciences Administration

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Abstract

Experiments were undertaken to test the hypothesis that the p24/CD9 cell surface molecule (originally detected on acute lymphoblastic leukemia cells) and the p21 protein encoded by the ras gene family are related. Immunoprecipitation and guanine nucleotide binding assays conducted with BA-2 and anti-p21/ras monoclonal antibodies showed no evidence that p24/CD9 is structurally or functionally related to p21/ras.

Original language	English (US)
Pages (from-to)	1565-1570
Number of pages	6
Journal	Leukemia research
Volume	9
Issue number	12
DOIs	https://doi.org/10.1016/0145-2126(85)90050-5
State	Published - 1985

Bibliographical note

Funding Information:
THE PRODUCTION and characterization of monoclonal antibodies recognizing cell surface molecules expressed on human lymphohematopoietic cells has been undertaken by numerous laboratories. Several years ago we produced a monoclonal antibody (designated BA-2) against human acute lymphoblastic leukemia (ALL) cells that recognizes a 24-kilodalton (kDa) cell surface molecule \[13\].E xtensive structural studies have revealed that p24 is a non-integral membrane molecule that does not contain detectable N-linked oligosaccharides, does contain covalently attached fatty acid, and is non-covalently associated with a homologous molecule designated p26 \[17, 19\]. Subsequent to our initial study \[13\], we and others reported that BA-2 and additional anti-p24 monoclonal antibodies are broadly reactive with normal and malignant lymphohematopoietic and nonlympbohematopoietic cells \[2, 3, 5, 9, 10, 12, 14, 21\]. The p24 molecule (hereafter referred to as *This work was supported by CA-21737, CA-25097, CA-31685 and RR-05385 from the National Institutes of Health, and the Leukemia Research Fund (formerly the Leukemia Task Force). T.W.L. is a Scholar of the Leukemia Society of America. .4bhreviations: ALL, acute lymphoblastic leukemia; kDa, kilodahon; HaMuSI/; Harvey routine sarcoma virus; KiMuSV, Kirsten murine sarcoma virus; RIP, radioimmunoprecipita-tion: SDS-PAGE, sodium dodecyt sulfate-polyacrylamide gel clectrophoresis; GTP, guanosine triphosphate.

Keywords

guanine nucleotide binding
p21/ras
p24/CD9

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title = "No evidence for structural or functional identity between p24/CD9 and p21/ras",

abstract = "Experiments were undertaken to test the hypothesis that the p24/CD9 cell surface molecule (originally detected on acute lymphoblastic leukemia cells) and the p21 protein encoded by the ras gene family are related. Immunoprecipitation and guanine nucleotide binding assays conducted with BA-2 and anti-p21/ras monoclonal antibodies showed no evidence that p24/CD9 is structurally or functionally related to p21/ras.",

keywords = "guanine nucleotide binding, p21/ras, p24/CD9",

author = "{Tucker W.}, {Le Bien} and Kersey, {John H.}",

note = "Funding Information: THE PRODUCTION and characterization of monoclonal antibodies recognizing cell surface molecules expressed on human lymphohematopoietic cells has been undertaken by numerous laboratories. Several years ago we produced a monoclonal antibody (designated BA-2) against human acute lymphoblastic leukemia (ALL) cells that recognizes a 24-kilodalton (kDa) cell surface molecule \[13\].E xtensive structural studies have revealed that p24 is a non-integral membrane molecule that does not contain detectable N-linked oligosaccharides, does contain covalently attached fatty acid, and is non-covalently associated with a homologous molecule designated p26 \[17, 19\]. Subsequent to our initial study \[13\], we and others reported that BA-2 and additional anti-p24 monoclonal antibodies are broadly reactive with normal and malignant lymphohematopoietic and nonlympbohematopoietic cells \[2, 3, 5, 9, 10, 12, 14, 21\]. The p24 molecule (hereafter referred to as *This work was supported by CA-21737, CA-25097, CA-31685 and RR-05385 from the National Institutes of Health, and the Leukemia Research Fund (formerly the Leukemia Task Force). T.W.L. is a Scholar of the Leukemia Society of America. .4bhreviations: ALL, acute lymphoblastic leukemia; kDa, kilodahon; HaMuSI/; Harvey routine sarcoma virus; KiMuSV, Kirsten murine sarcoma virus; RIP, radioimmunoprecipita-tion: SDS-PAGE, sodium dodecyt sulfate-polyacrylamide gel clectrophoresis; GTP, guanosine triphosphate.",

year = "1985",

doi = "10.1016/0145-2126(85)90050-5",

language = "English (US)",

volume = "9",

pages = "1565--1570",

journal = "Leukemia research",

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PY - 1985

Y1 - 1985

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KW - guanine nucleotide binding

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KW - p24/CD9

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No evidence for structural or functional identity between p24/CD9 and p21/ras

Abstract

Bibliographical note

Keywords

UN SDGs

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