Non-equivalent interactions between amino-terminal domains of neighboring λ integrase protomers direct holliday junction resolution

Sang Yeol Lee; Marta Radman-Livaja; David Warren; Hideki Aihara; Tom Ellenberger; Arthur Landy

doi:10.1016/j.jmb.2004.10.068

Non-equivalent interactions between amino-terminal domains of neighboring λ integrase protomers direct holliday junction resolution

Sang Yeol Lee, Marta Radman-Livaja, David Warren, Hideki Aihara, Tom Ellenberger, Arthur Landy

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The bacteriophage λ site-specific recombinase (Int), in contrast to other family members such as Cre and Flp, has an amino-terminal domain that binds "arm-type" DNA sequences different and distant from those involved in strand exchange. This defining feature of the heterobivalent recombinases confers a directionality and regulation that is unique among all recombination pathways. We show that the amino-terminal domain is not a simple "accessory" element, as originally thought, but rather is incorporated into the core of the recombination mechanism, where it is well positioned to exert its profound effects. The results reveal an unexpected pattern of intermolecular interactions between the amino-terminal domain of one protomer and the linker region of its neighbor within the tetrameric Int complex and provide insights into those features distinguishing an "active" from an "inactive" pair of Ints during Holliday junction resolution.

Original language	English (US)
Pages (from-to)	475-485
Number of pages	11
Journal	Journal of Molecular Biology
Volume	345
Issue number	3
DOIs	https://doi.org/10.1016/j.jmb.2004.10.068
State	Published - Jan 21 2005
Externally published	Yes

Bibliographical note

Funding Information:
We thank Christine Lank for technical assistance, Joan Boyles for manuscript preparation, and members of the Landy and Ellenberger laboratories for helpful discussions. This work was supported by National Institutes of Health grants GM62723 and GM33928 to A.L. and GM59902 to T.E.

Keywords

E. coli bacteriophage
multimeric complexes
protein-DNA interactions
protein-protein interactions
site-specific recombination

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title = "Non-equivalent interactions between amino-terminal domains of neighboring λ integrase protomers direct holliday junction resolution",

abstract = "The bacteriophage λ site-specific recombinase (Int), in contrast to other family members such as Cre and Flp, has an amino-terminal domain that binds {"}arm-type{"} DNA sequences different and distant from those involved in strand exchange. This defining feature of the heterobivalent recombinases confers a directionality and regulation that is unique among all recombination pathways. We show that the amino-terminal domain is not a simple {"}accessory{"} element, as originally thought, but rather is incorporated into the core of the recombination mechanism, where it is well positioned to exert its profound effects. The results reveal an unexpected pattern of intermolecular interactions between the amino-terminal domain of one protomer and the linker region of its neighbor within the tetrameric Int complex and provide insights into those features distinguishing an {"}active{"} from an {"}inactive{"} pair of Ints during Holliday junction resolution.",

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note = "Funding Information: We thank Christine Lank for technical assistance, Joan Boyles for manuscript preparation, and members of the Landy and Ellenberger laboratories for helpful discussions. This work was supported by National Institutes of Health grants GM62723 and GM33928 to A.L. and GM59902 to T.E. ",

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AU - Warren, David

AU - Aihara, Hideki

AU - Ellenberger, Tom

AU - Landy, Arthur

N1 - Funding Information: We thank Christine Lank for technical assistance, Joan Boyles for manuscript preparation, and members of the Landy and Ellenberger laboratories for helpful discussions. This work was supported by National Institutes of Health grants GM62723 and GM33928 to A.L. and GM59902 to T.E.

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Non-equivalent interactions between amino-terminal domains of neighboring λ integrase protomers direct holliday junction resolution

Abstract

Bibliographical note

Keywords

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