Novel insertion sequence-like element is982 in lactococci

Weizhu Yu; Igor Mierau; Astrid Mars; Eric Johnson; Gary Dunny; Larry L. McKay

doi:10.1006/plas.1995.1023

Novel insertion sequence-like element is982 in lactococci

Weizhu Yu, Igor Mierau, Astrid Mars, Eric Johnson, Gary Dunny, Larry L. McKay

Microbiology

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

A novel insertion sequence-like (IS) element, designated IS982, was found on the lactose plasmid, pSK11L, from Lactococcus lactis subsp. cremoris SK11 and was located between the origin of replication and the oligopeptide transport gene cluster. The 1003-base pair (bp) IS982 was flanked by 18-bp perfect inverted repeats. IS982 contained an open reading frame encoding a putative transposase of 296 amino acids. An almost identical IS-like element (99% DNA sequence identity) was cloned and partially sequenced from the chromosome of Lactococcus lactis subsp. cremoris Wg2 with 17-bp perfect inverted repeats. Southern analysis indicated that in 12 lactococcal strains examined, IS982 was present with copy numbers ranging from 1 to at least 20. IS982 displayed sequence homology to the putative IS element RSBst-α from Bacillus stearothermophilus CU21. IS982 and RSBst-α were not related to other known insertion sequences and may represent a new family of IS elements.

Original language	English (US)
Pages (from-to)	218-225
Number of pages	8
Journal	Plasmid
Volume	33
Issue number	3
DOIs	https://doi.org/10.1006/plas.1995.1023
State	Published - May 1995

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title = "Novel insertion sequence-like element is982 in lactococci",

abstract = "A novel insertion sequence-like (IS) element, designated IS982, was found on the lactose plasmid, pSK11L, from Lactococcus lactis subsp. cremoris SK11 and was located between the origin of replication and the oligopeptide transport gene cluster. The 1003-base pair (bp) IS982 was flanked by 18-bp perfect inverted repeats. IS982 contained an open reading frame encoding a putative transposase of 296 amino acids. An almost identical IS-like element (99% DNA sequence identity) was cloned and partially sequenced from the chromosome of Lactococcus lactis subsp. cremoris Wg2 with 17-bp perfect inverted repeats. Southern analysis indicated that in 12 lactococcal strains examined, IS982 was present with copy numbers ranging from 1 to at least 20. IS982 displayed sequence homology to the putative IS element RSBst-α from Bacillus stearothermophilus CU21. IS982 and RSBst-α were not related to other known insertion sequences and may represent a new family of IS elements.",

author = "Weizhu Yu and Igor Mierau and Astrid Mars and Eric Johnson and Gary Dunny and McKay, {Larry L.}",

year = "1995",

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language = "English (US)",

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T1 - Novel insertion sequence-like element is982 in lactococci

AU - Yu, Weizhu

AU - Mierau, Igor

AU - Mars, Astrid

AU - Johnson, Eric

AU - Dunny, Gary

AU - McKay, Larry L.

PY - 1995/5

Y1 - 1995/5

N2 - A novel insertion sequence-like (IS) element, designated IS982, was found on the lactose plasmid, pSK11L, from Lactococcus lactis subsp. cremoris SK11 and was located between the origin of replication and the oligopeptide transport gene cluster. The 1003-base pair (bp) IS982 was flanked by 18-bp perfect inverted repeats. IS982 contained an open reading frame encoding a putative transposase of 296 amino acids. An almost identical IS-like element (99% DNA sequence identity) was cloned and partially sequenced from the chromosome of Lactococcus lactis subsp. cremoris Wg2 with 17-bp perfect inverted repeats. Southern analysis indicated that in 12 lactococcal strains examined, IS982 was present with copy numbers ranging from 1 to at least 20. IS982 displayed sequence homology to the putative IS element RSBst-α from Bacillus stearothermophilus CU21. IS982 and RSBst-α were not related to other known insertion sequences and may represent a new family of IS elements.

AB - A novel insertion sequence-like (IS) element, designated IS982, was found on the lactose plasmid, pSK11L, from Lactococcus lactis subsp. cremoris SK11 and was located between the origin of replication and the oligopeptide transport gene cluster. The 1003-base pair (bp) IS982 was flanked by 18-bp perfect inverted repeats. IS982 contained an open reading frame encoding a putative transposase of 296 amino acids. An almost identical IS-like element (99% DNA sequence identity) was cloned and partially sequenced from the chromosome of Lactococcus lactis subsp. cremoris Wg2 with 17-bp perfect inverted repeats. Southern analysis indicated that in 12 lactococcal strains examined, IS982 was present with copy numbers ranging from 1 to at least 20. IS982 displayed sequence homology to the putative IS element RSBst-α from Bacillus stearothermophilus CU21. IS982 and RSBst-α were not related to other known insertion sequences and may represent a new family of IS elements.

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DO - 10.1006/plas.1995.1023

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VL - 33

SP - 218

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JO - Plasmid

JF - Plasmid

IS - 3

ER -

Novel insertion sequence-like element is982 in lactococci

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