Novel method for determination of 5-hydroxymethylfurfural in glucose biphasic hydrolyzate by UV spectroscopy

Liu Yang, Yu Huan Liu, Rong Sheng Ruan, Yun Pu Wang, Jin Li Ma, Shi Tao Liu, Jin Sheng Zhang, Yi Qin Wan, Hong Peng

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The concentration of 5-hydroxymethlfurfural (5-HMF) in glucose biphasic hydrolysate is rapidly determined by UV spectroscopy. With 5-HMF as the standard sample and the distilled water as the controlling blank instead of water-dimethylsulfoxide (DMSO) biphasic solution, the absorbance of 5-HMF at 284 nm is analyzed. The results show that a favorable linear relationship between the absorbance and the concentration of 5-HMF can be obtained with good reproducibility when the concentration of 5-HMF is within the range of 0-0.10 mmol/L. This method is suitable for rapid and accurate determination of 5-HMF.

Original languageEnglish (US)
Pages (from-to)92-95
Number of pages4
JournalXiandai Huagong/Modern Chemical Industry
Volume31
Issue number10
StatePublished - Oct 1 2011

Keywords

  • 5-hydroxymethlfurfural (5-HMF)
  • Glucose biphasic hydrolysate
  • UV spectroscopy

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