TY - JOUR
T1 - N2-ethyldeoxyguanosine as a potential biomarker for assessing effects of alcohol consumption on DNA
AU - Balbo, Silvia
AU - Hashibe, Mia
AU - Gundy, Sarolta
AU - Brennan, Paul
AU - Canova, Cristina
AU - Simonato, Lorenzo
AU - Merletti, Franco
AU - Richiardi, Lorenzo
AU - Agudo, Antonio
AU - Castellsagué, Xavier
AU - Znaor, Ariana
AU - Talamini, Renato
AU - Bencko, Vladimir
AU - Holcátová, Ivana
AU - Wang, Mingyao
AU - Hecht, Stephen S.
AU - Boffetta, Paolo
PY - 2008/11
Y1 - 2008/11
N2 - Head and neck cancers are causally related to alcohol consumption, but the underlying mechanisms are unclear. Ethanol is metabolized to acetaldehyde, an experimental carcinogen. Quantitation of the major DNA adduct of acetaldehyde, N2-ethylidenedeoxyguanosine, in human tissues could help to elucidate the mechanism of alcohol carcinogenicity. We applied a quantitative method for the analysis of this adduct, measured as the NaBH3CN reduction product N2-ethyldeoxyguanosine (N2-ethyl-dGuo) by liquid chromatography-electrospray ionization-tandem mass spectrometry-selected reaction monitoring, on DNA (0.04 ± 0.03 mg) isolated from blood collected from control subjects recruited from two studies conducted in different areas of Europe between 1999 and 2005. The group selected from the first study (n = 127) included alcohol drinkers and abstainers while the group from the second study (n = 50) included only heavy drinkers. N 2-ethyl-dGuo was detected in all DNA samples. After adjusting for potential confounders, in the first study, drinkers showed a higher level of N2-ethyld-Guo (5,270 ± 8,770 fmol/Mmol dGuo) compared with nondrinkers (2,690 ± 3040 fmol/Mmol dGuo; P = 0.04). A significant trend according to dose was observed in both studies (P = 0.02 and 0.04, respectively). Taking into account the amount of alcohol consumption, adduct levels were higher in younger compared with older subjects (P = 0.01), whereas no differences were observed comparing men with women. These results show the feasibility of quantifying N2-ethyl-dGuo in small-volume blood samples and are consistent with the hypothesis that ethanol contributes to carcinogenesis through DNA adducts formation.
AB - Head and neck cancers are causally related to alcohol consumption, but the underlying mechanisms are unclear. Ethanol is metabolized to acetaldehyde, an experimental carcinogen. Quantitation of the major DNA adduct of acetaldehyde, N2-ethylidenedeoxyguanosine, in human tissues could help to elucidate the mechanism of alcohol carcinogenicity. We applied a quantitative method for the analysis of this adduct, measured as the NaBH3CN reduction product N2-ethyldeoxyguanosine (N2-ethyl-dGuo) by liquid chromatography-electrospray ionization-tandem mass spectrometry-selected reaction monitoring, on DNA (0.04 ± 0.03 mg) isolated from blood collected from control subjects recruited from two studies conducted in different areas of Europe between 1999 and 2005. The group selected from the first study (n = 127) included alcohol drinkers and abstainers while the group from the second study (n = 50) included only heavy drinkers. N 2-ethyl-dGuo was detected in all DNA samples. After adjusting for potential confounders, in the first study, drinkers showed a higher level of N2-ethyld-Guo (5,270 ± 8,770 fmol/Mmol dGuo) compared with nondrinkers (2,690 ± 3040 fmol/Mmol dGuo; P = 0.04). A significant trend according to dose was observed in both studies (P = 0.02 and 0.04, respectively). Taking into account the amount of alcohol consumption, adduct levels were higher in younger compared with older subjects (P = 0.01), whereas no differences were observed comparing men with women. These results show the feasibility of quantifying N2-ethyl-dGuo in small-volume blood samples and are consistent with the hypothesis that ethanol contributes to carcinogenesis through DNA adducts formation.
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U2 - 10.1158/1055-9965.EPI-08-0117
DO - 10.1158/1055-9965.EPI-08-0117
M3 - Article
C2 - 18990745
AN - SCOPUS:55849125829
SN - 1055-9965
VL - 17
SP - 3026
EP - 3032
JO - Cancer Epidemiology Biomarkers and Prevention
JF - Cancer Epidemiology Biomarkers and Prevention
IS - 11
ER -