TY - JOUR
T1 - Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from ovine- and bovine-associated staphylococcus aureus
AU - Lee, Peter K
AU - Kreiswirth, Barry N.
AU - Deringer, James R.
AU - Projan, Steven J.
AU - Eisner, William
AU - Smith-Keiling, Beverly L
AU - Carlson, Eunice
AU - Novick, Richard P.
AU - Schlievert, Patrick M.
PY - 1992/6
Y1 - 1992/6
N2 - Toxic shock syndrome toxin (TSST) 1 was purified from ovine (TSST-ovine) and bovine (TSST-bovine) mastitis-associated Staphylococcus aureus. These toxins were previously reported to have molecular weights identical to that of human TSST-1. However, TSST-ovine was reported as having an isoelectric point (pi) of 8.5, whereas TSST-bovine has the same pi (7.2) as TSST-1. Nucleotide sequence analysis revealed that TSST-bovine was identical to TSST-1 and that TSST-ovine had 14 nucleotide differences that changed 9 amino acid residues. Only 1 nucleotide difference, at position 514, was predicted to cause an amino acid charge difference, as glutamic acid at position 132 of TSST-1 was changed to lysine in TSST-ovine. Like TSST-1, TSST-ovine was mitogenic, but unlike TSST-1, it was not pyrogenic, was unable to enhance endotoxic shock, and was unable to induce TSS in a rabbit model. Also, TSST-ovine was less reactive to certain monoclonal antibodies raised against TSST-1.
AB - Toxic shock syndrome toxin (TSST) 1 was purified from ovine (TSST-ovine) and bovine (TSST-bovine) mastitis-associated Staphylococcus aureus. These toxins were previously reported to have molecular weights identical to that of human TSST-1. However, TSST-ovine was reported as having an isoelectric point (pi) of 8.5, whereas TSST-bovine has the same pi (7.2) as TSST-1. Nucleotide sequence analysis revealed that TSST-bovine was identical to TSST-1 and that TSST-ovine had 14 nucleotide differences that changed 9 amino acid residues. Only 1 nucleotide difference, at position 514, was predicted to cause an amino acid charge difference, as glutamic acid at position 132 of TSST-1 was changed to lysine in TSST-ovine. Like TSST-1, TSST-ovine was mitogenic, but unlike TSST-1, it was not pyrogenic, was unable to enhance endotoxic shock, and was unable to induce TSS in a rabbit model. Also, TSST-ovine was less reactive to certain monoclonal antibodies raised against TSST-1.
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U2 - 10.1093/infdis/165.6.1056
DO - 10.1093/infdis/165.6.1056
M3 - Article
C2 - 1583323
AN - SCOPUS:0026562104
SN - 0022-1899
VL - 165
SP - 1056
EP - 1063
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -