Occurrence of 4-hydroxyalkenals in rat tissues determined as pentafluorobenzyl oxime derivatives by gas chromatography-mass spectrometry

Frederik J.G.M. van Kuijk, David W. Thomas, Robert J. Stephens, Edward A. Dratz

Research output: Contribution to journalArticlepeer-review

99 Scopus citations

Abstract

Malondialdehyde measurements have been the major tool for studying relationships between lipid peroxidation and tissue pathology. Recently, we presented a novel gas chromatography-mass spectrometry method for direct detection of phospholipid peroxides with picogram sensitivity based on transesterfication of phospholipids or triglycerides to form pentafluorobenzyl esters. Under some circumstances the reactive primary oxidation products break down. Therefore, we developed a convenient, high sensitivity method to detect more stable secondary lipid oxidation products, the 4-hydroxyalkenals. The method accomplishes a facile extraction of 4-hydroxynonenal from tissues by forming pentafluorobenzyl oxime derivatives to displace aldehydes from Schiff base linkages. 4-hydroxynonenal was found in heart, liver, adrenal, and testis from rats and was detected to the 10-100 pg level by the current method.

Original languageEnglish (US)
Pages (from-to)144-149
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume139
Issue number1
DOIs
StatePublished - Aug 29 1986

Bibliographical note

Funding Information:
This work was supported by the Netherlands Organization for the Advancement of Pure Research (ZWO) (to FJGMvK), and grants from the USA National Eye Institute (to EAD and RJS). We thank Prof. Dr. H. Esterbauer, Institute for Biochemistry, University of Graz, Graz, Austria, for the generous gift of 4-hydroxyalkenals, and many useful discussions.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

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