TY - CHAP
T1 - Oligomerization of retrovirus integrases
AU - Grandgenett, Duane P.
AU - Aihara, Hideki
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Integration of the reverse-transcribed viral cDNA into the host’s genome is a critical step in the lifecycle of all retroviruses. Retrovirus integration is carried out by integrase (IN), a virus-encoded enzyme that forms an oligomeric ‘intasome’ complex with both ends of the linear viral DNA to catalyze their concerted insertions into the backbones of the host’s DNA. IN also forms a complex with host proteins, which guides the intasome to the host’s chromosome. Recent structural studies have revealed remarkable diversity as well as conserved features among the architectures of the intasome assembly from different genera of retroviruses. This chapter will review how IN oligomerizes to achieve its function, with particular focus on alpharetrovirus including the avian retrovirus Rous sarcoma virus. Another chapter (Craigie) will focus on the structure and function of IN from HIV-1.
AB - Integration of the reverse-transcribed viral cDNA into the host’s genome is a critical step in the lifecycle of all retroviruses. Retrovirus integration is carried out by integrase (IN), a virus-encoded enzyme that forms an oligomeric ‘intasome’ complex with both ends of the linear viral DNA to catalyze their concerted insertions into the backbones of the host’s DNA. IN also forms a complex with host proteins, which guides the intasome to the host’s chromosome. Recent structural studies have revealed remarkable diversity as well as conserved features among the architectures of the intasome assembly from different genera of retroviruses. This chapter will review how IN oligomerizes to achieve its function, with particular focus on alpharetrovirus including the avian retrovirus Rous sarcoma virus. Another chapter (Craigie) will focus on the structure and function of IN from HIV-1.
KW - Alpharetrovirus
KW - Avian sarcoma/leukemia (ASLV)
KW - Betaretrovirus
KW - Bromodomain and extra-terminal (BET)
KW - C-terminal domain (CTD)
KW - Catalytic core domain (CCD)
KW - Concerted integration
KW - Conserved intasome core (CIC)
KW - Cryo electron microscopy (EM)
KW - Crystal structure
KW - D,D(35)E motif
KW - FAcilitates Chromatin Transcription (FACT)
KW - Human immunodeficiency virus type 1 (HIV-1)
KW - Intasome
KW - Integrase (IN)
KW - Lens epithelium-derived growth factor (LEDGF/p75)
KW - Lentivirus
KW - Mouse mammary tumor virus (MMTV)
KW - N-terminal domain (NTD)
KW - Preintegration complex (PIC)
KW - Prototype foamy virus (PFV)
KW - Retrovirus
KW - Rous sarcoma virus (RSV)
KW - Spumavirus
KW - Stable synaptic complex (SSC)
KW - Strand transfer complex (STC)
KW - Strand transfer inhibitor (STI)
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UR - http://www.scopus.com/inward/citedby.url?scp=85048667670&partnerID=8YFLogxK
U2 - 10.1007/978-981-10-8456-0_10
DO - 10.1007/978-981-10-8456-0_10
M3 - Chapter
C2 - 29900499
AN - SCOPUS:85048667670
T3 - Subcellular Biochemistry
SP - 211
EP - 243
BT - Subcellular Biochemistry
PB - Springer New York
ER -