On the function of pentameric phospholamban: Ion channel or storage form?

Lucia Becucci, Alessandro Cembran, Christine B. Karim, David D. Thomas, Rolando Guidelli, Jiali Gao, Gianluigi Veglia

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Phospholamban (PLN) isan integralmembrane protein that inhibits the sarcoplasmic reticulumCa2+-ATPase, thereby regulating muscle contractility.We report a combined electrochemical and theoretical study demonstrating that the pentameric PLN does not possess channel activity for conducting chloride or calciumions across the lipidmembrane. This suggests that the pentameric configuration of PLN primarily serves as a storage form for the regulatory function of muscle relaxation by the PLN monomer.

Original languageEnglish (US)
Pages (from-to)L60-L62
JournalBiophysical journal
Volume96
Issue number10
DOIs
StatePublished - 2009

Bibliographical note

Funding Information:
This work was supported by grants from the National Institutes of Health to G.V. (GM64742, HL80081, GM072701), D.D.T. (GM27906), J.G. (GM46736), J.G. from the Office of Naval Research, and R.G. from Ente Cassa di Risparmio (Firenze, Italy). All computations were performed on computers at PNNL through an EMSL Grand Challenge grant.

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