On the function of pentameric phospholamban: Ion channel or storage form?

Lucia Becucci; Alessandro Cembran; Christine B. Karim; David D. Thomas; Rolando Guidelli; Jiali Gao; Gianluigi Veglia

doi:10.1016/j.bpj.2009.03.013

On the function of pentameric phospholamban: Ion channel or storage form?

Lucia Becucci, Alessandro Cembran, Christine B. Karim, David D. Thomas, Rolando Guidelli, Jiali Gao, Gianluigi Veglia

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Abstract

Phospholamban (PLN) isan integralmembrane protein that inhibits the sarcoplasmic reticulumCa²⁺-ATPase, thereby regulating muscle contractility.We report a combined electrochemical and theoretical study demonstrating that the pentameric PLN does not possess channel activity for conducting chloride or calciumions across the lipidmembrane. This suggests that the pentameric configuration of PLN primarily serves as a storage form for the regulatory function of muscle relaxation by the PLN monomer.

Original language	English (US)
Pages (from-to)	L60-L62
Journal	Biophysical journal
Volume	96
Issue number	10
DOIs	https://doi.org/10.1016/j.bpj.2009.03.013
State	Published - 2009

Bibliographical note

Funding Information:
This work was supported by grants from the National Institutes of Health to G.V. (GM64742, HL80081, GM072701), D.D.T. (GM27906), J.G. (GM46736), J.G. from the Office of Naval Research, and R.G. from Ente Cassa di Risparmio (Firenze, Italy). All computations were performed on computers at PNNL through an EMSL Grand Challenge grant.

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title = "On the function of pentameric phospholamban: Ion channel or storage form?",

abstract = "Phospholamban (PLN) isan integralmembrane protein that inhibits the sarcoplasmic reticulumCa2+-ATPase, thereby regulating muscle contractility.We report a combined electrochemical and theoretical study demonstrating that the pentameric PLN does not possess channel activity for conducting chloride or calciumions across the lipidmembrane. This suggests that the pentameric configuration of PLN primarily serves as a storage form for the regulatory function of muscle relaxation by the PLN monomer.",

author = "Lucia Becucci and Alessandro Cembran and Karim, {Christine B.} and Thomas, {David D.} and Rolando Guidelli and Jiali Gao and Gianluigi Veglia",

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AU - Becucci, Lucia

AU - Cembran, Alessandro

AU - Karim, Christine B.

AU - Thomas, David D.

AU - Guidelli, Rolando

AU - Gao, Jiali

AU - Veglia, Gianluigi

N1 - Funding Information: This work was supported by grants from the National Institutes of Health to G.V. (GM64742, HL80081, GM072701), D.D.T. (GM27906), J.G. (GM46736), J.G. from the Office of Naval Research, and R.G. from Ente Cassa di Risparmio (Firenze, Italy). All computations were performed on computers at PNNL through an EMSL Grand Challenge grant.

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On the function of pentameric phospholamban: Ion channel or storage form?

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