Oncogenic JAK2 V617F causes PD-L1 expression, mediating immune escape in myeloproliferative neoplasms

Alessandro Prestipino, Alica J. Emhardt, Konrad Aumann, David O'Sullivan, Sivahari P. Gorantla, Sandra Duquesne, Wolfgang Melchinger, Lukas Braun, Slavica Vuckovic, Melanie Boerries, Hauke Busch, Sebastian Halbach, Sandra Pennisi, Teresa Poggio, Petya Apostolova, Pia Veratti, Michael Hettich, Gabriele Niedermann, Mark Bartholomä, Khalid ShoumariyehJonas S. Jutzi, Julius Wehrle, Christine Dierks, Heiko Becker, Annette Schmitt-Graeff, Marie Follo, Dietmar Pfeifer, Jan Rohr, Sebastian Fuchs, Stephan Ehl, Frederike A. Hartl, Susana Minguet, Cornelius Miething, Florian H. Heidel, Nicolaus Kröger, Ioanna Triviai, Tilman Brummer, Jürgen Finke, Anna L. Illert, Eliana Ruggiero, Chiara Bonini, Justus Duyster, Heike L. Pahl, Steven W. Lane, Geoffrey R. Hill, Bruce R. Blazar, Nikolas Von Bubnoff, Erika L. Pearce, Robert Zeiser

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59 Scopus citations

Abstract

Recent evidence has revealed that oncogenic mutations may confer immune escape. A better understanding of how an oncogenic mutation affects immunosuppressive programmed death ligand 1 (PD-L1) expression may help in developing new therapeutic strategies. We show that oncogenic JAK2 (Janus kinase 2) activity caused STAT3 (signal transducer and activator of transcription 3) and STAT5 phosphorylation, which enhanced PD-L1 promoter activity and PD-L1 protein expression in JAK2 V617F -mutant cells, whereas blockade of JAK2 reduced PD-L1 expression in myeloid JAK2 V617F -mutant cells. PD-L1 expression was higher on primary cells isolated from patients with JAK2 V617F -myeloproliferative neoplasms (MPNs) compared to healthy individuals and declined upon JAK2 inhibition. JAK2 V617F mutational burden, pSTAT3, and PD-L1 expression were highest in primary MPN patient-derived monocytes, megakaryocytes, and platelets. PD-1 (programmed death receptor 1) inhibition prolonged survival in human MPN xenograft and primary murine MPN models. This effect was dependent on T cells. Mechanistically, PD-L1 surface expression in JAK2 V617F -mutant cells affected metabolism and cell cycle progression of T cells. In summary, we report that in MPN, constitutive JAK2/STAT3/STAT5 activation, mainly in monocytes, megakaryocytes, and platelets, caused PD-L1-mediated immune escape by reducing T cell activation, metabolic activity, and cell cycle progression. The susceptibility of JAK2 V617F -mutant MPN to PD-1 targeting paves the way for immunomodulatory approaches relying on PD-1 inhibition.

Original languageEnglish (US)
Article numbereaam7729
JournalScience Translational Medicine
Volume10
Issue number429
DOIs
StatePublished - Feb 21 2018

Bibliographical note

Funding Information:
This study was supported by European Research Council Consolidator grant 681012 GvHDCure to R.Z., Deutsche Forschungsgemeinschaft (DFG) (Heisenberg Professorship ZE872/3-2 to R.Z., SFB1160/P14 to R.Z., P1 to S.E., SFB850/Z1 to H.B. and M.B., TRR167-NeuroMAC to R.Z., EXC-306 to H.B., MI1942/2-1 to S.M., and DKH 111764 to N.v.B.), Excellence Initiative (DFG, GSC-4, Spemann Graduate School of Biology and Medicine) scholarship to J.S.J., MSCA-ITN-2015-ETN-ALKATRAS (A.L.I.), NIH R01 CA72669 and P01 AI056299 to B.R.B., Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie (Germany) (BMBF) to M.B. and S.E. (e:Med research), BMBF 01EO1303, DeCaRe (FKZ 01ZX1409B), DFG 2508/4-1 (N.v.B.), and Max Planck Society (E.L.P.). E.R. was supported by a fellowship by Associazione Italiana per la Ricerca sul Cancro cofunded by the European Union.

Publisher Copyright:
Copyright © 2018 The Authors, some rights reserved.

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