TY - JOUR
T1 - Oncogenic KrasG12D causes myeloproliferation via NLRP3 inflammasome activation
AU - Hamarsheh, Shaima’a
AU - Osswald, Lena
AU - Saller, Benedikt S.
AU - Unger, Susanne
AU - De Feo, Donatella
AU - Vinnakota, Janaki Manoja
AU - Konantz, Martina
AU - Uhl, Franziska M.
AU - Becker, Heiko
AU - Lübbert, Michael
AU - Shoumariyeh, Khalid
AU - Schürch, Christoph
AU - Andrieux, Geoffroy
AU - Venhoff, Nils
AU - Schmitt-Graeff, Annette
AU - Duquesne, Sandra
AU - Pfeifer, Dietmar
AU - Cooper, Matthew A.
AU - Lengerke, Claudia
AU - Boerries, Melanie
AU - Duyster, Justus
AU - Niemeyer, Charlotte M.
AU - Erlacher, Miriam
AU - Blazar, Bruce R.
AU - Becher, Burkard
AU - Groß, Olaf
AU - Brummer, Tilman
AU - Zeiser, Robert
N1 - Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Oncogenic Ras mutations occur in various leukemias. It was unclear if, besides the direct transforming effect via constant RAS/MEK/ERK signaling, an inflammation-related effect of KRAS contributes to the disease. Here, we identify a functional link between oncogenic KrasG12D and NLRP3 inflammasome activation in murine and human cells. Mice expressing active KrasG12D in the hematopoietic system developed myeloproliferation and cytopenia, which is reversed in KrasG12D mice lacking NLRP3 in the hematopoietic system. Therapeutic IL-1-receptor blockade or NLRP3-inhibition reduces myeloproliferation and improves hematopoiesis. Mechanistically, KrasG12D-RAC1 activation induces reactive oxygen species (ROS) production causing NLRP3 inflammasome-activation. In agreement with our observations in mice, patient-derived myeloid leukemia cells exhibit KRAS/RAC1/ROS/NLRP3/IL-1β axis activity. Our findings indicate that oncogenic KRAS not only act via its canonical oncogenic driver function, but also enhances the activation of the pro-inflammatory RAC1/ROS/NLRP3/IL-1β axis. This paves the way for a therapeutic approach based on immune modulation via NLRP3 blockade in KRAS-mutant myeloid malignancies.
AB - Oncogenic Ras mutations occur in various leukemias. It was unclear if, besides the direct transforming effect via constant RAS/MEK/ERK signaling, an inflammation-related effect of KRAS contributes to the disease. Here, we identify a functional link between oncogenic KrasG12D and NLRP3 inflammasome activation in murine and human cells. Mice expressing active KrasG12D in the hematopoietic system developed myeloproliferation and cytopenia, which is reversed in KrasG12D mice lacking NLRP3 in the hematopoietic system. Therapeutic IL-1-receptor blockade or NLRP3-inhibition reduces myeloproliferation and improves hematopoiesis. Mechanistically, KrasG12D-RAC1 activation induces reactive oxygen species (ROS) production causing NLRP3 inflammasome-activation. In agreement with our observations in mice, patient-derived myeloid leukemia cells exhibit KRAS/RAC1/ROS/NLRP3/IL-1β axis activity. Our findings indicate that oncogenic KRAS not only act via its canonical oncogenic driver function, but also enhances the activation of the pro-inflammatory RAC1/ROS/NLRP3/IL-1β axis. This paves the way for a therapeutic approach based on immune modulation via NLRP3 blockade in KRAS-mutant myeloid malignancies.
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U2 - 10.1038/s41467-020-15497-1
DO - 10.1038/s41467-020-15497-1
M3 - Article
C2 - 32246016
AN - SCOPUS:85083041601
SN - 2041-1723
VL - 11
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 1659
ER -