Oncolytic adenoviruses: Design, generation, and experimental procedures

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Oncolytic adenoviruses are designed to take advantage of the virus' native ability to replicate in cancer cells to induce oncolysis. Subsequently, the released viral progeny spread and kill the neighboring cancer cells. These characteristics, together with the ability of adenovirus to infect a broad spectrum of cells, its well understood replication machinery, and relative ease of manufacture have led to the intensive use of adenovirus as an anticancer agent. This unit describes cloning strategies, procedures to turn the intended design into virus, and quality analyses of resultant adenoviral vectors. Most of these procedures were optimized especially for oncolytic adenoviral vectors.

Original languageEnglish (US)
Article number2.14
JournalCurrent Protocols in Human Genetics
Issue numberSUPPL.78
DOIs
StatePublished - Jan 1 2013

Keywords

  • Adenovirus
  • Amplification
  • Cloning
  • Design
  • Oncolytic
  • Production
  • Purification
  • Strategy

Fingerprint

Dive into the research topics of 'Oncolytic adenoviruses: Design, generation, and experimental procedures'. Together they form a unique fingerprint.

Cite this