Opsonic requirements for the uptake of cryptococcus neoformans by human polymorphonuclear leukocytes and monocytes

Scott F Davies, Dennis P. Clifford, John R. Hoidal, John E. Repine

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Chemiluminescence was used to determine opsonic requirements for interaction of Cryptococcus neoformans with human peripheral blood polymorphonuclear leukocytes (PMNLs) and monocytes (MNs). Peak chemiluminescence (mean ± SD) was 172 ± 25 x 103 for PMNLs and 50 ± 13 x 103 for MNs with 20070 pooled normal human serum vs. 10 ± 3 x 103 for PMNLs and 6 ± 1 x 103 for MNs without serum (P < 0.005 for each comparison). The nature of the serum requirement was investigated; alternative pathway serum complement components were necessary. The alternative pathway was activated directly by C. neoformans without the need for specific antibody. Chemiluminescence provided a quantitative and reproducible assay of the interaction of human phagocytes with C. neoformans and may be useful in studying opsonic requirements of other fungi.

Original languageEnglish (US)
Pages (from-to)870-874
Number of pages5
JournalJournal of Infectious Diseases
Volume145
Issue number6
DOIs
StatePublished - Jun 1982

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