Optimization of a microfluidic device for diffusion-based extraction of DMSO from a cell suspension

K. K. Fleming Glass, E. K. Longmire, A. Hubel

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26 Scopus citations

Abstract

This study considers the use of a two stream microfluidic device for extraction of dimethyl sulfoxide (DMSO) from a cryopreserved cell suspension. The DMSO diffuses from a cell suspension stream into a neighboring wash stream flowing in parallel. The model of Fleming et al. [K.K. Fleming, E.K. Longmire, A. Hubel, Numerical characterization of diffusion-based extraction in a cell-laden flow through a microfluidic channel, Journal of Biomechanical Engineering 129 (2007) 703-711] is employed to determine and discuss optimal geometry and operating conditions for a case requiring removal of 95% DMSO from suspension streams with volumetric flow rates up to 2.5 ml/min. The effects of Peclet number, flow rate fraction, and cell volume fraction are analyzed, and expansion of the analysis to other applications is discussed.

Original languageEnglish (US)
Pages (from-to)5749-5757
Number of pages9
JournalInternational Journal of Heat and Mass Transfer
Volume51
Issue number23-24
DOIs
StatePublished - Nov 2008

Bibliographical note

Funding Information:
The authors gratefully acknowledge support for this work from the National Blood Foundation, the National Institutes of Health (R21EB004857), and the University of Minnesota Graduate School. Also, the authors would like to thank Dr. Clara Mata and Dr. David McKenna for their valuable input to this study.

Keywords

  • Cell suspension
  • Channel flow
  • Diffusion
  • Optimization

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