Sodium iodide symporter (NIS) reporter gene imaging is an excellent technology for noninvasive cell fate determination in living animals unless the NIS-transduced cells reside in perigastric organs such as the spleen, liver, diaphragm, omentum, pancreas, perigastric lymph nodes or perigastric tumor deposits. Here we report that orally administered barium sulfate enhances CT definition of the stomach, masks background gamma ray emissions from the stomach and enhances signal detection from radiotracer uptake in NIS-transduced organs.
Bibliographical noteFunding Information:
We thank Suzanne Greiner (Mayo Clinic) for her veterinary expertise, Michael K O’Connor (Radiology, Mayo Clinic) for critical review of the manuscript, Tracy Decklever and Brad Kemp (Radiology, Mayo Clinic) of the Mayo Clinic Small Animal Imaging Core. This work was supported by the Mayo Foundation and grants from the National Institutes of Health (R01CA129193, R01CA100634, R01CA129966).
- barium sulfate
- oral contrast
- reporter gene