Organic nitrogen addition suppresses fungal richness and alters community composition in temperate forest soils

Lauren C. Cline, Julia A. Huggins, Sarah E. Hobbie, Peter G. Kennedy

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Human-driven increases in bioavailable nitrogen over the last century have revealed the critical need to understand how nitrogen availability influences terrestrial ecosystems. In contrast to widespread evidence that increasing inorganic nitrogen strongly influences the diversity and composition of plant and microbial communities, the effect of organic nitrogen addition, the predominant form of nitrogen in soils, remains less clear. In this study, we conducted a field experiment manipulating both the amount and composition of soil organic nitrogen present in soil fungal in-growth bags, followed by molecular characterization of fungal communities after a four-month incubation. Saprotrophic and ectomycorrhizal fungi readily colonized experimental in-growth bags, indicating a broad overlap in the fundamental niches of these two functional guilds. Increases in labile forms of organic nitrogen resulted in sharp declines in species richness and diversity across fungal guilds, as well as notable shifts in fungal community composition. The relative abundance of fungi classified as molds and yeasts peaked where organic nitrogen was both high and most labile, whereas slower-growing saprotrophic and ectomycorrhizal fungi were more abundant in the non-amended treatments. Taken together, our results indicate that similar to inorganic nitrogen, increasing the amount of organic nitrogen can dramatically alter the richness and composition of fungal communities in temperate forest soils.

Original languageEnglish (US)
Pages (from-to)222-230
Number of pages9
JournalSoil Biology and Biochemistry
Volume125
DOIs
StatePublished - Oct 2018

Bibliographical note

Funding Information:
This work was supported by an NSF RUBC Postdoctoral Fellowship ( 1611856 ) to L. Cline and NSF-DEB grant ( 1554375 ) to P. Kennedy. We thank Lauren Sullivan for experimental design advice, Chris Fernandez for his advice classifying ectomycorrhizal fungi as ruderal or slow-growing, and both Alex Tang and Amanda Certano for help deploying and harvesting in-growth bags as well as laboratory assistance. We are also grateful to Carla Rosenfeld, Shelly Rorer, Rachel King, Chris Walters, and Chris Buyarski for help with nitrogen analyses, as well as two anonymous reviewers and members of the Kennedy lab for helpful comments in improving the presentation our results and conclusions.

Publisher Copyright:
© 2018 Elsevier Ltd

Keywords

  • Ectomycorrhizae
  • Nitrogen
  • Saprotroph
  • Soil fungi

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