Overexpression of defense response genes in transgenic wheat enhances resistance to Fusarium head blight

Caroline A. Mackintosh; Janet Lewis; Lorien E. Radmer; Sanghyun Shin; Shane J. Heinen; Lisa A. Smith; Meagen N. Wyckoff; Ruth Dill-Macky; Conrad K. Evans; Sasha Kravchenko; Gerald D. Baldridge; Richard J. Zeyen; Gary J. Muehlbauer

doi:10.1007/s00299-006-0265-8

Overexpression of defense response genes in transgenic wheat enhances resistance to Fusarium head blight

Caroline A. Mackintosh, Janet Lewis, Lorien E. Radmer, Sanghyun Shin, Shane J. Heinen, Lisa A. Smith, Meagen N. Wyckoff, Ruth Dill-Macky, Conrad K. Evans, Sasha Kravchenko, Gerald D. Baldridge, Richard J. Zeyen, Gary J. Muehlbauer

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Abstract

Fusarium head blight (FHB) of wheat, caused by Fusarium graminearum and other Fusarium species, is a major disease problem for wheat production worldwide. To combat this problem, large-scale breeding efforts have been established. Although progress has been made through standard breeding approaches, the level of resistance attained is insufficient to withstand epidemic conditions. Genetic engineering provides an alternative approach to enhance the level of resistance. Many defense response genes are induced in wheat during F. graminearum infection and may play a role in reducing FHB. The objectives of this study were (1) to develop transgenic wheat overexpressing the defense response genes α-1-purothionin, thaumatin-like protein 1 (tlp-1), and β-1,3-glucanase; and (2) to test the resultant transgenic wheat lines against F. graminearum infection under greenhouse and field conditions. Using the wheat cultivar Bobwhite, we developed one, two, and four lines carrying the α-1-purothionin, tlp-1, and β-1,3-glucanase transgenes, respectively, that had statistically significant reductions in FHB severity in greenhouse evaluations. We tested these seven transgenic lines under field conditions for percent FHB disease severity, deoxynivalenol (DON) mycotoxin accumulation, and percent visually scabby kernels (VSK). Six of the seven lines differed from the nontransgenic parental Bobwhite line for at least one of the disease traits. A β-1,3-glucanase transgenic line had enhanced resistance, showing lower FHB severity, DON concentration, and percent VSK compared to Bobwhite. Taken together, the results showed that overexpression of defense response genes in wheat could enhance the FHB resistance in both greenhouse and field conditions.

Original language	English (US)
Pages (from-to)	479-488
Number of pages	10
Journal	Plant Cell Reports
Volume	26
Issue number	4
DOIs	https://doi.org/10.1007/s00299-006-0265-8
State	Published - Apr 2007

Bibliographical note

Funding Information:
Acknowledgements We are most grateful to Dr. Tom Clemente and Shirley Sato of the University of Nebraska and to Drs. Ann Blechl, and Pat Okubara USDA-ARS, Albany, CA for their advise and assistance in generating the transgenic wheat plants. We are also grateful to Drs. Blechl, Okubara and Kent McCue and for providing the pKM1 and pUBK BglII transformation constructs. We thank Dr. John Mundy of the Carlsberg Research Laboratory, Copenhagen, Denmark and Dr. David Collinge of the Department of Plant Pathology, Royal Veterinary and Agricultural University, Copenhagen, Denmark for providing barley β- 1,3-glucanase and tlp-1 cDNAs, respectively. We also thank Dr. Peter Quail of the Plant Gene Expression Center, University of California at Berkeley for providing the pAHC25 and pAHC17 plasmids. We are indebted to Keith Hansen, Adam Dobberfuhl, Dong Tuong, Sarah Jutila, Alissa Cyrus, John Wiersma, Amar Elakkad, Karen Wennberg and Yanhong Dong for excellent technical assistance. Sanghyun Shin was supported by the Korea Research Foundation (KRF-2005-000-10035). This work was supported by grants from the U.S. Wheat and Barley Scab Initiative, Minnesota State Scab Initiative, and Minnesota Wheat Research and Promotion Council.

Keywords

Fusarium graminearum
Fusarium head blight
Transgenic wheat
Triticum aestivum
Wheat

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Mackintosh, C. A., Lewis, J., Radmer, L. E., Shin, S., Heinen, S. J., Smith, L. A., Wyckoff, M. N., Dill-Macky, R., Evans, C. K., Kravchenko, S., Baldridge, G. D., Zeyen, R. J., & Muehlbauer, G. J. (2007). Overexpression of defense response genes in transgenic wheat enhances resistance to Fusarium head blight. Plant Cell Reports, 26(4), 479-488. https://doi.org/10.1007/s00299-006-0265-8

Mackintosh, CA, Lewis, J, Radmer, LE, Shin, S, Heinen, SJ, Smith, LA, Wyckoff, MN, Dill-Macky, R, Evans, CK, Kravchenko, S, Baldridge, GD, Zeyen, RJ & Muehlbauer, GJ 2007, 'Overexpression of defense response genes in transgenic wheat enhances resistance to Fusarium head blight', Plant Cell Reports, vol. 26, no. 4, pp. 479-488. https://doi.org/10.1007/s00299-006-0265-8

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title = "Overexpression of defense response genes in transgenic wheat enhances resistance to Fusarium head blight",

abstract = "Fusarium head blight (FHB) of wheat, caused by Fusarium graminearum and other Fusarium species, is a major disease problem for wheat production worldwide. To combat this problem, large-scale breeding efforts have been established. Although progress has been made through standard breeding approaches, the level of resistance attained is insufficient to withstand epidemic conditions. Genetic engineering provides an alternative approach to enhance the level of resistance. Many defense response genes are induced in wheat during F. graminearum infection and may play a role in reducing FHB. The objectives of this study were (1) to develop transgenic wheat overexpressing the defense response genes α-1-purothionin, thaumatin-like protein 1 (tlp-1), and β-1,3-glucanase; and (2) to test the resultant transgenic wheat lines against F. graminearum infection under greenhouse and field conditions. Using the wheat cultivar Bobwhite, we developed one, two, and four lines carrying the α-1-purothionin, tlp-1, and β-1,3-glucanase transgenes, respectively, that had statistically significant reductions in FHB severity in greenhouse evaluations. We tested these seven transgenic lines under field conditions for percent FHB disease severity, deoxynivalenol (DON) mycotoxin accumulation, and percent visually scabby kernels (VSK). Six of the seven lines differed from the nontransgenic parental Bobwhite line for at least one of the disease traits. A β-1,3-glucanase transgenic line had enhanced resistance, showing lower FHB severity, DON concentration, and percent VSK compared to Bobwhite. Taken together, the results showed that overexpression of defense response genes in wheat could enhance the FHB resistance in both greenhouse and field conditions.",

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author = "Mackintosh, {Caroline A.} and Janet Lewis and Radmer, {Lorien E.} and Sanghyun Shin and Heinen, {Shane J.} and Smith, {Lisa A.} and Wyckoff, {Meagen N.} and Ruth Dill-Macky and Evans, {Conrad K.} and Sasha Kravchenko and Baldridge, {Gerald D.} and Zeyen, {Richard J.} and Muehlbauer, {Gary J.}",

note = "Funding Information: Acknowledgements We are most grateful to Dr. Tom Clemente and Shirley Sato of the University of Nebraska and to Drs. Ann Blechl, and Pat Okubara USDA-ARS, Albany, CA for their advise and assistance in generating the transgenic wheat plants. We are also grateful to Drs. Blechl, Okubara and Kent McCue and for providing the pKM1 and pUBK BglII transformation constructs. We thank Dr. John Mundy of the Carlsberg Research Laboratory, Copenhagen, Denmark and Dr. David Collinge of the Department of Plant Pathology, Royal Veterinary and Agricultural University, Copenhagen, Denmark for providing barley β- 1,3-glucanase and tlp-1 cDNAs, respectively. We also thank Dr. Peter Quail of the Plant Gene Expression Center, University of California at Berkeley for providing the pAHC25 and pAHC17 plasmids. We are indebted to Keith Hansen, Adam Dobberfuhl, Dong Tuong, Sarah Jutila, Alissa Cyrus, John Wiersma, Amar Elakkad, Karen Wennberg and Yanhong Dong for excellent technical assistance. Sanghyun Shin was supported by the Korea Research Foundation (KRF-2005-000-10035). This work was supported by grants from the U.S. Wheat and Barley Scab Initiative, Minnesota State Scab Initiative, and Minnesota Wheat Research and Promotion Council.",

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AU - Lewis, Janet

AU - Radmer, Lorien E.

AU - Shin, Sanghyun

AU - Heinen, Shane J.

AU - Smith, Lisa A.

AU - Wyckoff, Meagen N.

AU - Dill-Macky, Ruth

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AU - Kravchenko, Sasha

AU - Baldridge, Gerald D.

AU - Zeyen, Richard J.

AU - Muehlbauer, Gary J.

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N2 - Fusarium head blight (FHB) of wheat, caused by Fusarium graminearum and other Fusarium species, is a major disease problem for wheat production worldwide. To combat this problem, large-scale breeding efforts have been established. Although progress has been made through standard breeding approaches, the level of resistance attained is insufficient to withstand epidemic conditions. Genetic engineering provides an alternative approach to enhance the level of resistance. Many defense response genes are induced in wheat during F. graminearum infection and may play a role in reducing FHB. The objectives of this study were (1) to develop transgenic wheat overexpressing the defense response genes α-1-purothionin, thaumatin-like protein 1 (tlp-1), and β-1,3-glucanase; and (2) to test the resultant transgenic wheat lines against F. graminearum infection under greenhouse and field conditions. Using the wheat cultivar Bobwhite, we developed one, two, and four lines carrying the α-1-purothionin, tlp-1, and β-1,3-glucanase transgenes, respectively, that had statistically significant reductions in FHB severity in greenhouse evaluations. We tested these seven transgenic lines under field conditions for percent FHB disease severity, deoxynivalenol (DON) mycotoxin accumulation, and percent visually scabby kernels (VSK). Six of the seven lines differed from the nontransgenic parental Bobwhite line for at least one of the disease traits. A β-1,3-glucanase transgenic line had enhanced resistance, showing lower FHB severity, DON concentration, and percent VSK compared to Bobwhite. Taken together, the results showed that overexpression of defense response genes in wheat could enhance the FHB resistance in both greenhouse and field conditions.

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KW - Fusarium head blight

KW - Transgenic wheat

KW - Triticum aestivum

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Overexpression of defense response genes in transgenic wheat enhances resistance to Fusarium head blight

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