TY - JOUR
T1 - Oxidation of oxyphenbutazone by sheep vesicular gland microsomes and lipoxygenase
AU - Portoghese, Philip S.
AU - Svanborg, Kerstin
AU - Samuelsson, Bengt
N1 - Funding Information:
A. Pfeiffer Memorial Research Fellowship.
PY - 1975/4/7
Y1 - 1975/4/7
N2 - Oxyphenbutazone was oxidized when incubated with acetone powder prepared from sheep vesicular gland microsomes or with lipoxygenase at pH 4 or 5. Oxidation also occurred at pH 8 or 9, if arachidonate or linoleate was added to either of the incubation mixtures. The oxygenated product was found to be identical with 4-hydroxyoxyphenbutazone, which was synthesized and analyzed by gas liquid chromatography and mass spectrometry. The oxygenated compound was not an inhibitor of prostaglandin biosynthesis.
AB - Oxyphenbutazone was oxidized when incubated with acetone powder prepared from sheep vesicular gland microsomes or with lipoxygenase at pH 4 or 5. Oxidation also occurred at pH 8 or 9, if arachidonate or linoleate was added to either of the incubation mixtures. The oxygenated product was found to be identical with 4-hydroxyoxyphenbutazone, which was synthesized and analyzed by gas liquid chromatography and mass spectrometry. The oxygenated compound was not an inhibitor of prostaglandin biosynthesis.
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U2 - 10.1016/S0006-291X(75)80447-5
DO - 10.1016/S0006-291X(75)80447-5
M3 - Article
C2 - 236749
AN - SCOPUS:0016651259
SN - 0006-291X
VL - 63
SP - 748
EP - 755
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -