Oxyphenbutazone was oxidized when incubated with acetone powder prepared from sheep vesicular gland microsomes or with lipoxygenase at pH 4 or 5. Oxidation also occurred at pH 8 or 9, if arachidonate or linoleate was added to either of the incubation mixtures. The oxygenated product was found to be identical with 4-hydroxyoxyphenbutazone, which was synthesized and analyzed by gas liquid chromatography and mass spectrometry. The oxygenated compound was not an inhibitor of prostaglandin biosynthesis.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Apr 7 1975|
Bibliographical noteFunding Information:
A. Pfeiffer Memorial Research Fellowship.