Oxidized NiTi surfaces enhance differentiation of osteoblast-like cells

A. Michiardi, E. Engel, C. Aparicio, J. A. Planell, F. J. Gil

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A new oxidation treatment (OT) on NiTi shape memory alloys was developed in a previous work. This OT treatment significantly decreases Ni ion release into the exterior medium, and therefore is thought to be beneficial for NiTi cytocompatibility. As to confirm this expectation, the in vitro response of MG63 osteoblast-like cells cultured on untreated and oxidized NiTi surfaces was studied. An adhesion test at 1, 4, and 8 h of incubation was performed. Statistical differences were evidenced at 1 h of adhesion depending on the surface treatment and chemical composition of the substrate. However, at larger times of study, there were no statistically significant differences between untreated and oxidized surfaces. The proliferation test (until 9 days) showed that untreated and oxidized NiTi surfaces are not cytotoxic for MG63 cells. The differences of adhesion at short times did not affect the proliferation of MG63 cells. However, after 48 h of stimulation with ascorbic acid and dexamethasone, the MG63 cells cultured on oxidized surfaces showed higher alkaline phosphatase activity and osteocalcin levels. The improvement of osteoblast differentiation due to OT treatment could accelerate bone formation, and, therefore, could allow earlier loading of NiTi devices used in dental and orthopedic applications.

Original languageEnglish (US)
Pages (from-to)108-114
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume85
Issue number1
DOIs
StatePublished - Apr 1 2008

Keywords

  • Cell adhesion
  • Cell differentiation
  • Cell proliferation
  • Nickel-titanium alloy
  • Osteoblast
  • in vitro test

Fingerprint

Dive into the research topics of 'Oxidized NiTi surfaces enhance differentiation of osteoblast-like cells'. Together they form a unique fingerprint.

Cite this