P-limitation drives changes in DOM production by aquatic bacteria

Seth K. Thompson, James B. Cotner

Research output: Contribution to journalArticlepeer-review

Abstract

Heterotrophic bacteria are key biogeochemical regulators in freshwater systems. Through both decomposition and production of organic matter, bacteria link multiple biogeochemical cycles together. While there has been a significant amount of work done on understanding the role of microbes in the aquatic carbon cycle, important linkages with other biogeochemical cycles will require more information about how organic matter transformations impact other nutrients, such as phosphorus. In this study, we conducted a culture-based laboratory experiment to examine the production of dissolved organic matter (DOM) by heterotrophic bacteria under varied nutrient conditions. In addition to quantifying the production of dissolved organic carbon (DOC), we also measured the production of dissolved organic phosphorus (DOP) and characterized the microbially produced organic matter using optical properties. Results demonstrated that measurable amounts of DOC and DOP were produced by heterotrophic bacteria under nutrient regimes ranging from carbon-limitation to strong phosphorus-limitation. Additionally, optical characterization of DOM revealed that the organic matter produced by bacteria grown under high phosphorus conditions was highly aromatic with similar optical properties to terrestrially derived organic matter. Overall, these findings suggest that heterotrophic bacteria can be important producers of organic matter in freshwaters and that continued trends of increased nutrient concentrations (eutrophication) may fundamentally change the composition of microbially produced organic matter in freshwater systems.

Original languageEnglish (US)
Pages (from-to)35-46
Number of pages12
JournalAquatic Microbial Ecology
Volume85
DOIs
StatePublished - 2020

Bibliographical note

Funding Information:
Acknowledgements. The authors thank Sara Crader for her help in culturing and collecting samples and Andrea Little for her technical support on the project. This work was funded by the Itasca Graduate Student Fellowship and Moos Graduate Fellowship awarded to S.K.T. by the College of Biological Sciences at the University of Minnesota. The strains used in this study were isolated and characterized with funding from the National Science Foundation to J.B.C. (IOS Grant #1257571.

Keywords

  • Carbon
  • Dissolved organic matter
  • Heterotrophic bacteria
  • Microbes in biogeochemical cycling
  • Phosphorus

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