Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance

W. E. Scott; B. P. Weegman; J. Ferrer-Fabrega; S. A. Stein; T. Anazawa; V. A. Kirchner; M. D. Rizzari; J. Stone; S. Matsumoto; B. E. Hammer; A. N. Balamurugan; L. S. Kidder; T. M. Suszynski; E. S. Avgoustiniatos; S. G. Stone; L. A. Tempelman; D. E.R. Sutherland; B. J. Hering; K. K. Papas

doi:10.1016/j.transproceed.2010.05.091

Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance

W. E. Scott, B. P. Weegman, J. Ferrer-Fabrega, S. A. Stein, T. Anazawa, V. A. Kirchner, M. D. Rizzari, J. Stone, S. Matsumoto, B. E. Hammer, A. N. Balamurugan, L. S. Kidder, T. M. Suszynski, E. S. Avgoustiniatos, S. G. Stone, L. A. Tempelman, D. E.R. Sutherland, B. J. Hering, K. K. Papas

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Background: Islet transplantation is a promising treatment for type 1 diabetes. Due to a shortage of suitable human pancreata, high cost, and the large dose of islets presently required for long-term diabetes reversal; it is important to maximize viable islet yield. Traditional methods of pancreas preservation have been identified as suboptimal due to insufficient oxygenation. Enhanced oxygen delivery is a key area of improvement. In this paper, we explored improved oxygen delivery by persufflation (PSF), ie, vascular gas perfusion. Methods: Human pancreata were obtained from brain-dead donors. Porcine pancreata were procured by en bloc viscerectomy from heparinized donation after cardiac death donors and were either preserved by either two-layer method (TLM) or PSF. Following procurement, organs were transported to a 1.5-T magnetic resonance (MR) system for ³¹P nuclear magnetic resonance spectroscopy to investigate their bioenergetic status by measuring the ratio of adenosine triphosphate to inorganic phosphate (ATP:P_i) and for assessing PSF homogeneity by MRI. Results: Human and porcine pancreata can be effectively preserved by PSF. MRI showed that pancreatic tissue was homogeneously filled with gas. TLM can effectively raise ATP:P_i levels in rat pancreata but not in larger porcine pancreata. ATP:P_i levels were almost undetectable in porcine organs preserved with TLM. When human or porcine organs were preserved by PSF, ATP:P_i was elevated to levels similar to those observed in rat pancreata. Conclusion: The methods developed for human and porcine pancreas PSF homogeneously deliver oxygen throughout the organ. This elevates ATP levels during preservation and may improve islet isolation outcomes while enabling the use of marginal donors, thus expanding the usable donor pool.

Original language	English (US)
Pages (from-to)	2011-2015
Number of pages	5
Journal	Transplantation proceedings
Volume	42
Issue number	6
DOIs	https://doi.org/10.1016/j.transproceed.2010.05.091
State	Published - Jul 2010

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1016/j.transproceed.2010.05.091

OpenUrl availability

Full text

Cite this

Scott, W. E., Weegman, B. P., Ferrer-Fabrega, J., Stein, S. A., Anazawa, T., Kirchner, V. A., Rizzari, M. D., Stone, J., Matsumoto, S., Hammer, B. E., Balamurugan, A. N., Kidder, L. S., Suszynski, T. M., Avgoustiniatos, E. S., Stone, S. G., Tempelman, L. A., Sutherland, D. E. R., Hering, B. J., & Papas, K. K. (2010). Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance. Transplantation proceedings, 42(6), 2011-2015. https://doi.org/10.1016/j.transproceed.2010.05.091

Scott, WE, Weegman, BP, Ferrer-Fabrega, J, Stein, SA, Anazawa, T, Kirchner, VA, Rizzari, MD, Stone, J, Matsumoto, S, Hammer, BE, Balamurugan, AN, Kidder, LS, Suszynski, TM, Avgoustiniatos, ES, Stone, SG, Tempelman, LA, Sutherland, DER, Hering, BJ & Papas, KK 2010, 'Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance', Transplantation proceedings, vol. 42, no. 6, pp. 2011-2015. https://doi.org/10.1016/j.transproceed.2010.05.091

@article{15892b2a3082434c9d78060817924902,

title = "Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance",

abstract = "Background: Islet transplantation is a promising treatment for type 1 diabetes. Due to a shortage of suitable human pancreata, high cost, and the large dose of islets presently required for long-term diabetes reversal; it is important to maximize viable islet yield. Traditional methods of pancreas preservation have been identified as suboptimal due to insufficient oxygenation. Enhanced oxygen delivery is a key area of improvement. In this paper, we explored improved oxygen delivery by persufflation (PSF), ie, vascular gas perfusion. Methods: Human pancreata were obtained from brain-dead donors. Porcine pancreata were procured by en bloc viscerectomy from heparinized donation after cardiac death donors and were either preserved by either two-layer method (TLM) or PSF. Following procurement, organs were transported to a 1.5-T magnetic resonance (MR) system for 31P nuclear magnetic resonance spectroscopy to investigate their bioenergetic status by measuring the ratio of adenosine triphosphate to inorganic phosphate (ATP:Pi) and for assessing PSF homogeneity by MRI. Results: Human and porcine pancreata can be effectively preserved by PSF. MRI showed that pancreatic tissue was homogeneously filled with gas. TLM can effectively raise ATP:Pi levels in rat pancreata but not in larger porcine pancreata. ATP:Pi levels were almost undetectable in porcine organs preserved with TLM. When human or porcine organs were preserved by PSF, ATP:Pi was elevated to levels similar to those observed in rat pancreata. Conclusion: The methods developed for human and porcine pancreas PSF homogeneously deliver oxygen throughout the organ. This elevates ATP levels during preservation and may improve islet isolation outcomes while enabling the use of marginal donors, thus expanding the usable donor pool.",

author = "Scott, {W. E.} and Weegman, {B. P.} and J. Ferrer-Fabrega and Stein, {S. A.} and T. Anazawa and Kirchner, {V. A.} and Rizzari, {M. D.} and J. Stone and S. Matsumoto and Hammer, {B. E.} and Balamurugan, {A. N.} and Kidder, {L. S.} and Suszynski, {T. M.} and Avgoustiniatos, {E. S.} and Stone, {S. G.} and Tempelman, {L. A.} and Sutherland, {D. E.R.} and Hering, {B. J.} and Papas, {K. K.}",

year = "2010",

month = jul,

doi = "10.1016/j.transproceed.2010.05.091",

language = "English (US)",

volume = "42",

pages = "2011--2015",

journal = "Transplantation proceedings",

issn = "0041-1345",

publisher = "Elsevier USA",

number = "6",

}

TY - JOUR

T1 - Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance

AU - Scott, W. E.

AU - Weegman, B. P.

AU - Ferrer-Fabrega, J.

AU - Stein, S. A.

AU - Anazawa, T.

AU - Kirchner, V. A.

AU - Rizzari, M. D.

AU - Stone, J.

AU - Matsumoto, S.

AU - Hammer, B. E.

AU - Balamurugan, A. N.

AU - Kidder, L. S.

AU - Suszynski, T. M.

AU - Avgoustiniatos, E. S.

AU - Stone, S. G.

AU - Tempelman, L. A.

AU - Sutherland, D. E.R.

AU - Hering, B. J.

AU - Papas, K. K.

PY - 2010/7

Y1 - 2010/7

N2 - Background: Islet transplantation is a promising treatment for type 1 diabetes. Due to a shortage of suitable human pancreata, high cost, and the large dose of islets presently required for long-term diabetes reversal; it is important to maximize viable islet yield. Traditional methods of pancreas preservation have been identified as suboptimal due to insufficient oxygenation. Enhanced oxygen delivery is a key area of improvement. In this paper, we explored improved oxygen delivery by persufflation (PSF), ie, vascular gas perfusion. Methods: Human pancreata were obtained from brain-dead donors. Porcine pancreata were procured by en bloc viscerectomy from heparinized donation after cardiac death donors and were either preserved by either two-layer method (TLM) or PSF. Following procurement, organs were transported to a 1.5-T magnetic resonance (MR) system for 31P nuclear magnetic resonance spectroscopy to investigate their bioenergetic status by measuring the ratio of adenosine triphosphate to inorganic phosphate (ATP:Pi) and for assessing PSF homogeneity by MRI. Results: Human and porcine pancreata can be effectively preserved by PSF. MRI showed that pancreatic tissue was homogeneously filled with gas. TLM can effectively raise ATP:Pi levels in rat pancreata but not in larger porcine pancreata. ATP:Pi levels were almost undetectable in porcine organs preserved with TLM. When human or porcine organs were preserved by PSF, ATP:Pi was elevated to levels similar to those observed in rat pancreata. Conclusion: The methods developed for human and porcine pancreas PSF homogeneously deliver oxygen throughout the organ. This elevates ATP levels during preservation and may improve islet isolation outcomes while enabling the use of marginal donors, thus expanding the usable donor pool.

AB - Background: Islet transplantation is a promising treatment for type 1 diabetes. Due to a shortage of suitable human pancreata, high cost, and the large dose of islets presently required for long-term diabetes reversal; it is important to maximize viable islet yield. Traditional methods of pancreas preservation have been identified as suboptimal due to insufficient oxygenation. Enhanced oxygen delivery is a key area of improvement. In this paper, we explored improved oxygen delivery by persufflation (PSF), ie, vascular gas perfusion. Methods: Human pancreata were obtained from brain-dead donors. Porcine pancreata were procured by en bloc viscerectomy from heparinized donation after cardiac death donors and were either preserved by either two-layer method (TLM) or PSF. Following procurement, organs were transported to a 1.5-T magnetic resonance (MR) system for 31P nuclear magnetic resonance spectroscopy to investigate their bioenergetic status by measuring the ratio of adenosine triphosphate to inorganic phosphate (ATP:Pi) and for assessing PSF homogeneity by MRI. Results: Human and porcine pancreata can be effectively preserved by PSF. MRI showed that pancreatic tissue was homogeneously filled with gas. TLM can effectively raise ATP:Pi levels in rat pancreata but not in larger porcine pancreata. ATP:Pi levels were almost undetectable in porcine organs preserved with TLM. When human or porcine organs were preserved by PSF, ATP:Pi was elevated to levels similar to those observed in rat pancreata. Conclusion: The methods developed for human and porcine pancreas PSF homogeneously deliver oxygen throughout the organ. This elevates ATP levels during preservation and may improve islet isolation outcomes while enabling the use of marginal donors, thus expanding the usable donor pool.

UR - http://www.scopus.com/inward/record.url?scp=77955545753&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955545753&partnerID=8YFLogxK

U2 - 10.1016/j.transproceed.2010.05.091

DO - 10.1016/j.transproceed.2010.05.091

M3 - Article

C2 - 20692395

AN - SCOPUS:77955545753

SN - 0041-1345

VL - 42

SP - 2011

EP - 2015

JO - Transplantation proceedings

JF - Transplantation proceedings

IS - 6

ER -

Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance

Abstract

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this