Paradigm diagnostics salmonella indicator broth (PDX-SIB) for detection of salmonella on selected environmental surfaces

Alan Olstein, Leena Griffith, Joellen M Feirtag, Nicole Pearson

Research output: Contribution to journalArticlepeer-review

Abstract

The Paradigm Diagnostics Salmonella Indicator Broth (PDX-SIB) is intended as a single-step selective enrichment indicator broth to be used as a simple screening test for the presence of Salmonella spp. in environmental samples. This method permits the end user to avoid multistep sample processing to identify presumptively positive samples, as exemplified by standard U.S. reference methods. PDX-SIB permits the outgrowth of Salmonella while inhibiting the growth of competitive Gram-negative and -positive microflora. Growth of Salmonellapositive cultures results in a visual color change of the medium from purple to yellow when the sample is grown at 37 ± 1°C. Performance of PDX-SIB has been evaluated in five different categories: inclusivityexclusivity, methods comparison, ruggedness, lot-to-lot variability, and shelf stability. The inclusivity panel included 100 different Salmonella serovars, 98 of which were SIB-positive during the 30 to 48 h incubation period. The exclusivity panel included 33 different non-Salmonella microorganisms, 31 of which were SIB-negative during the incubation period. Methods comparison studies included four different surfaces: S. Newport on plastic, S. Anatum on sealed concrete, S. Abaetetuba on ceramic tile, and S. Typhimurium in the presence of 1 log excess of Citrobacter freundii. Results of the methods comparison studies demonstrated no statistical difference between the SIB method and the U.S. Food and Drug Administration-Bacteriological Analytical Manual reference method, as measured by the Mantel-Haenszel Chi-square test. Ruggedness studies demonstrated little variation in test results when SIB incubation temperatures were varied over a 34-40°C range. Lot-to-lot consistency results suggest no detectable differences in manufactured goods using two reference Salmonella serovars and one non-Salmonella microorganism.

Original languageEnglish (US)
Pages (from-to)404-412
Number of pages9
JournalJournal of AOAC International
Volume96
Issue number2
DOIs
StatePublished - Mar 1 2013

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