Two-polymer aqueous phase systems are described in which ribosomes selectively partition into one of the phases. One of the phase systems is used to determine rapidly and conveniently binding of thiostrepton and erythromycin to Escherichia coli ribosomes under equilibrium conditions.
Bibliographical noteFunding Information:
We are grateful to Dr. Harry Walter, who introduced us to the fascinating possibilities of two-polymer aqueous phase systems and who generously provided guidance. The third author gratefully acknowledges support by Research Career Development Award CA25258 from the National Cancer Institute.