Patterned optogenetic modulation of neurovascular and metabolic signals

Thomas J. Richner, Ryan Baumgartner, Sarah K. Brodnick, Mehdi Azimipour, Lisa A. Krugner-Higby, Kevin W. Eliceiri, Justin C. Williams, Ramin Pashaie

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


The hemodynamic and metabolic response of the cortex depends spatially and temporally on the activity of multiple cell types. Optogenetics enables specific cell types to be modulated with high temporal precision and is therefore an emerging method for studying neurovascular and neurometabolic coupling. Going beyond temporal investigations, we developed a microprojection system to apply spatial photostimulus patterns in vivo. We monitored vascular and metabolic fluorescence signals after photostimulation in Thy1-channelrhodopsin-2 mice. Cerebral arteries increased in diameter rapidly after photostimulation, while nearby veins showed a slower smaller response. The amplitude of the arterial response was depended on the area of cortex stimulated. The fluorescence signal emitted at 450/100 nm and excited with ultraviolet is indicative of reduced nicotinamide adenine dinucleotide, an endogenous fluorescent enzyme involved in glycolysis and the citric acid cycle. This fluorescence signal decreased quickly and transiently after optogenetic stimulation, suggesting that glucose metabolism is tightly locked to optogenetic stimulation. To verify optogenetic stimulation of the cortex, we used a transparent substrate microelectrode array to map cortical potentials resulting from optogenetic stimulation. Spatial optogenetic stimulation is a new tool for studying neurovascular and neurometabolic coupling.

Original languageEnglish (US)
Pages (from-to)140-147
Number of pages8
JournalJournal of Cerebral Blood Flow and Metabolism
Issue number1
StatePublished - Jan 10 2015

Bibliographical note

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  • cerebral hemodynamics
  • electrophysiology
  • intrinsic optical imaging
  • microscopy
  • neurovascular coupling


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