Patterns of coordinate down-regulation of ARE-containing transcripts following immune cell activation

Arvind Raghavan, Mohammed Dhalla, Tala Bakheet, Rachel L. Ogilvie, Irina A St. Louis, Khalid S.A. Khabar, Bryan R.G. Williams, Paul R Bohjanen

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

We evaluated the expression of over 900 AU-rich element (ARE)-containing transcripts in primary human T lymphocytes following stimulation with anti-CD3 and anti-CD28 antibodies and found that approximately 48% of these transcripts were regulated following T cell activation. We identified approximately 145 ARE-containing transcripts that were rapidly induced and then rapidly disappeared within 1 h after activation. Another 250 ARE-containing transcripts expressed in resting T cells were rapidly turned off within 30 min after activation. The rates of transcript disappearance correlated well with rapid mRNA decay measured following transcriptional arrest with actinomycin D. We identified a subset of ARE-containing transcripts that were rapidly induced following T cell activation that were also induced following lipopolysaccharide stimulation of THP-1 monocytes, and these transcripts exhibited rapid decay in both cell types. Our results suggest that ARE-mediated mRNA decay plays an important role in the precisely coordinated down-regulation of gene expression following immune cell activation.

Original languageEnglish (US)
Pages (from-to)1002-1013
Number of pages12
JournalGenomics
Volume84
Issue number6
DOIs
StatePublished - Dec 2004

Bibliographical note

Funding Information:
This work was supported by Grants AI49494 and AI52170 from the National Institutes of Health and by an award to the University of Minnesota Medical School under the Research Resources Program of the Howard Hughes Medical Institute.

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