TY - JOUR
T1 - Pax3 activation promotes the differentiation of mesenchymal stem cells toward the myogenic lineage
AU - Gang, Eun Ji
AU - Bosnakovski, Darko
AU - Simsek, Tugba
AU - To, Khai
AU - Perlingeiro, Rita C R
N1 - Publisher Copyright:
© 2008 Elsevier Inc. All rights reserved.
PY - 2008/5/1
Y1 - 2008/5/1
N2 - Mesenchymal stem cells (MSCs) residing within the bone marrow (BM) differentiate into multiple lineages, including fat, bone, and cartilage. Because MSCs are multipotent and have a great capacity to be expanded in vitro, these cells are an attractive candidate for clinical applications to repair or regenerate damaged tissues of mesenchymal origin. However, application of MSCs to muscle degenerative diseases has been hampered by the poor differentiation of MSCs into the muscle lineage. To date most methods require the presence of strong non-physiological agents, such as azacytidine. In the present study we explored the potential of Pax3, the master regulator of the embryonic myogenic program, to promote myogenic differentiation from MSCs. Our results clearly demonstrate that Pax3 promotes the differentiation of MSCs towards the myogenic lineage, which occurs at the expense of other mesenchymal lineages including fat, bone, and cartilage. This effect is cell type-selective since Pax3 overexpression in endothelial cells fails to promote myogenesis. These results highlight the potential of regulating transcriptional pathways to direct differentiation of adult stem cells.
AB - Mesenchymal stem cells (MSCs) residing within the bone marrow (BM) differentiate into multiple lineages, including fat, bone, and cartilage. Because MSCs are multipotent and have a great capacity to be expanded in vitro, these cells are an attractive candidate for clinical applications to repair or regenerate damaged tissues of mesenchymal origin. However, application of MSCs to muscle degenerative diseases has been hampered by the poor differentiation of MSCs into the muscle lineage. To date most methods require the presence of strong non-physiological agents, such as azacytidine. In the present study we explored the potential of Pax3, the master regulator of the embryonic myogenic program, to promote myogenic differentiation from MSCs. Our results clearly demonstrate that Pax3 promotes the differentiation of MSCs towards the myogenic lineage, which occurs at the expense of other mesenchymal lineages including fat, bone, and cartilage. This effect is cell type-selective since Pax3 overexpression in endothelial cells fails to promote myogenesis. These results highlight the potential of regulating transcriptional pathways to direct differentiation of adult stem cells.
KW - Bone
KW - Cartilage
KW - Cell differentiation
KW - Fat
KW - Mesenchymal stem cells
KW - Muscle
KW - Pax3
UR - http://www.scopus.com/inward/record.url?scp=42649130645&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42649130645&partnerID=8YFLogxK
U2 - 10.1016/j.yexcr.2008.02.016
DO - 10.1016/j.yexcr.2008.02.016
M3 - Article
C2 - 18395202
AN - SCOPUS:42649130645
SN - 0014-4827
VL - 314
SP - 1721
EP - 1733
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 8
ER -